Noncanonical cofactor biomimetics (NCBs) such as nicotinamide mononucleotide (NMN+) provide enhanced scalability for biomanufacturing. However, engineering enzymes to accept NCBs is difficult. Here, we establish a growth selection platform to evolve enzymes to utilize NMN+-based reducing power. This is based on an orthogonal, NMN+-dependent glycolytic pathway in
- Award ID(s):
- 1847705
- PAR ID:
- 10299528
- Date Published:
- Journal Name:
- Catalysts
- Volume:
- 10
- Issue:
- 8
- ISSN:
- 2073-4344
- Page Range / eLocation ID:
- 935
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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