Sap flow measurement is one of the most effective methods for quantifying plant water use.A better understanding of sap flow dynamics can aid in more efficient water and crop management, particularly under unpredictable rainfall patterns and water scarcity resulting from climate change. In addition to detecting infected plants, sap flow measurement helps select plant species that could better cope with hotter and drier conditions. There exist multiple methods to measure sap flow including heat balance, dyes and radiolabeled tracers. Heat sensor-based techniques are the most popular and commercially available to study plant hydraulics, even though most of them are invasive and associated with multiple kinds of errors. Heat-based methods are prone to errors due to misalignment of probes and wounding, despite all the advances in this technology. Among existing methods for measuring sap flow, nuclear magnetic resonance (NMR) is an appropriate non-invasive approach. However, there are challenges associated with applications of NMR to measure sap flow in trees or field crops, such as producing homogeneous magnetic field, bulkiness and poor portable nature of the instruments, and operational complexity. Nonetheless, various advances have been recently made that allow the manufacture of portable NMR tools for measuring sap flow in plants. The basic concept of the portal NMR tool is based on an external magnetic field to measure the sap flow and hence advances in magnet types and magnet arrangements (e.g., C-type, U-type, and Halbach magnets) are critical components of NMR-based sap flow measuring tools. Developing a non-invasive, portable and inexpensive NMR tool that can be easily used under field conditions would significantly improve our ability to monitor vegetation responses to environmental change.
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A double-ratio method to measure fast, slow and reverse sap flows
Abstract Sap velocity measurements are useful in fields ranging from plant water relations to hydrology at a variety of scales. Techniques based on pulses of heat are among the most common methods to measure sap velocity, but most lack ability to measure velocities across a wide range, including very high, very low and negative velocities (reverse flow). We propose a new method, the double-ratio method (DRM), which is robust across an unprecedented range of sap velocities and provides real-time estimates of the thermal diffusivity of wood. The DRM employs one temperature sensor upstream (proximal) and two sensors downstream (distal) to the source of heat. This facilitates several theoretical, heat-based approaches to quantifying sap velocity. We tested the DRM using whole-tree lysimetry in Eucalyptus cypellocarpa L.A.S. Johnson and found strong agreement across a wide range of velocities.
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- Award ID(s):
- 1951244
- PAR ID:
- 10314628
- Editor(s):
- Steppe, Kathy
- Date Published:
- Journal Name:
- Tree Physiology
- Volume:
- 41
- Issue:
- 12
- ISSN:
- 1758-4469
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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