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Abstract Human induced pluripotent stem cell derived brain organoids have shown great potential for studies of human brain development and neurological disorders. However, quantifying the evolution of the electrical properties of brain organoids during development is currently limited by the measurement techniques, which cannot provide long‐term stable 3D bioelectrical interfaces with developing brain organoids. Here, a cyborg brain organoid platform is reported, in which “tissue‐like” stretchable mesh nanoelectronics are designed to match the mechanical properties of brain organoids and to be folded by the organogenetic process of progenitor or stem cells, distributing stretchable electrode arrays across the 3D organoids. The tissue‐wide integrated stretchable electrode arrays show no interruption to brain organoid development, adapt to the volume and morphological changes during brain organoid organogenesis, and provide long‐term stable electrical contacts with neurons within brain organoids during development. The seamless and noninvasive coupling of electrodes to neurons enables long‐term stable, continuous recording and captures the emergence of single‐cell action potentials from early‐stage brain organoid development.
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Brain Organoids – expanding on understanding human brain development, schizophrenia and 'Phase Zero' therapies
Stem cell-derived brain organoids replicate important stages of the prenatal human brain development and combined with the induced pluripotent stem cells (iPSCs) technology offer an unprecedented model for investigating human neurodevelopmental diseases including schizophrenia and autism. I will discuss new insights into organoid-based model of schizophrenia and shed light on challenges and future applications of organoid disease model system. Studies of iPSC and cerebral organoids in combination with electrophysiology, 3D genomics and novel technologies such as nanophotonics/optogenomics, unravel potential applications in the search for new drug treatments and novel technologies such as nanophotonics/optogenomics for controlling and correcting the brain development.
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- Award ID(s):
- 2039190
- PAR ID:
- 10315618
- Date Published:
- Journal Name:
- 13th INAR conference and 7th International Conference
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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