Title: Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization
Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrrolidone/antibiotic/isoflavonoid thin films by the matrix-assisted pulsed laser evaporation (MAPLE) method as anti-adhesion barrier coatings, on biomedical surfaces for improved resistance to microbial colonization. The thin films were characterized by Fourier transform infrared spectroscopy, infrared microscopy, and scanning electron microscopy. In vitro biological assay tests were performed to evaluate the influence of the thin films on the development of biofilms formed by Gram-positive and Gram-negative bacterial strains. In vitro biocompatibility tests were assessed on human endothelial cells examined for up to five days of incubation, via qualitative and quantitative methods. The results of this study revealed that the laser-fabricated coatings are biocompatible and resistant to microbial colonization and biofilm formation, making them successful candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission. more »« less
Cristescu, Rodica; Narayan, Roger J.; Chrisey, Douglas B.
(, MRS Advances)
null
(Ed.)
Abstract Antimicrobial surface coatings function as a contact biocide and are extensively used to prevent the growth and transmission of pathogens on environmental surfaces. Currently, scientists and researchers are intensively working to develop antimicrobial, antiviral coating solutions that would efficiently impede/stop the contagion of COVID-19 via surface contamination. Herein we present a flavonoid-based antimicrobial surface coating fabricated by laser processing that has the potential to eradicate COVID-19 contact transmission. Quercetin-containing coatings showed better resistance to microbial colonization than antibiotic–containing ones.
Khlyustova, Alexandra; Kirsch, Mia; Ma, Xiaojing; Cheng, Yifan; Yang, Rong
(, Journal of Materials Chemistry B)
Due to the emergence of wide-spread infectious diseases, there is a heightened need for antimicrobial and/or antifouling coatings that can be used to prevent infection and transmission in a variety of applications, ranging from healthcare devices to public facilities. While antimicrobial coatings kill pathogenic bacteria upon contact with the surface, the antimicrobial function alone often lacks long-term effectiveness due to the accumulation of dead cells and their debris on the surface, thus reducing the performance of the coating over time. Therefore, it is desirable to develop coatings with the dual functions of antimicrobial efficacy and fouling resistance, in which antifouling coatings provide the added benefit of preventing the adhesion of dead cells and debris. Leveraging the outstanding antifouling properties of zwitterionic coatings, we synthesized copolymers with this antimicrobial-antifouling dual function by immobilizing lysozyme, a common antimicrobial enzyme, to the surface of a pyridinium-based zwitterionic copolymer. Specifically, poly(4-vinylpyridine- co -pentaflurophenyl methacrylate- co -divinyl benzene) [P(4VP-PFPMA-DVB)] thin films were synthesized by an all-dry vapor deposition technique, initiated Chemical Vapor Deposition, and derivatized using 1,3-propane sultone to obtain sulfobetaine moieties. Lysozyme, known to hydrolyze polysaccharides in the cell wall of Gram-positive bacteria, was immobilized by forming amide bonds with the copolymer coating via nucleophilic substitution of the pentafluorophenyl group. The antifouling and antibacterial performance of the novel lysozyme-zwitterionic coating was tested against Gram-positive Bacillus subtilis and Gram-negative Pseudomonas aeruginosa . A reduction in surface adhesion of 87% was achieved for P. aeruginosa , and of 75% for B. subtilis , when compared to a common poly(vinyl chloride) surface. The lysozyme-zwitterionic coating also deactivated 67% of surface-attached Gram-positive bacteria, B. subtilis . This novel dual-function material can produce anti -infection surfaces for medical devices and surgical tools, personal care products, and surfaces in public facilities.
We explored the potential of biomimetic thin films fabricated by means of matrix-assisted pulsed laser evaporation (MAPLE) for releasing combinations of active substances represented by flavonoids (quercetin dihydrate and resveratrol) and antifungal compounds (amphotericin B and voriconazole) embedded in a polyvinylpyrrolidone biopolymer; the antifungal activity of the film components was evaluated using in vitro microbiological assays. Thin films were deposited using a pulsed KrF* excimer laser source which were structurally characterized using atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). High-quality thin films with chemical structures similar to dropcast ones were created using an optimum laser fluence of ~80 mJ/cm2. Bioactive substances were included within the polymer thin films using the MAPLE technique. The results of the in vitro microbiology assay, which utilized a modified disk diffusion approach and were performed using two fungal strains (Candida albicans American Type Culture Collection (ATCC) 90028 and Candida parapsilosis American Type Culture Collection (ATCC) 22019), revealed that voriconazole was released in an active form from the polyvinylpyrrolidone matrix. The results of this study show that the MAPLE-deposited bioactive thin films have a promising potential for use in designing combination devices, such as drug delivery devices, and medical device surfaces with antifungal activity.
Soto-Garcia, Luis F.; Guerrero-Rodriguez, Ingrid D.; Hoang, Luu; Laboy-Segarra, Samantha Lauren; Phan, Ngan T.; Villafuerte, Enrique; Lee, Juhyun; Nguyen, Kytai T.
(, International Journal of Molecular Sciences)
Bacterial infection has traditionally been treated with antibiotics, but their overuse is leading to the development of antibiotic resistance. This may be mitigated by alternative approaches to prevent or treat bacterial infections without utilization of antibiotics. Among the alternatives is the use of photo-responsive antimicrobial nanoparticles and/or nanocomposites, which present unique properties activated by light. In this study, we explored the combined use of titanium oxide and polydopamine to create nanoparticles with photocatalytic and photothermal antibacterial properties triggered by visible or near-infrared light. Furthermore, as a proof-of-concept, these photo-responsive nanoparticles were combined with mussel-inspired catechol-modified hyaluronic acid hydrogels to form novel light-driven antibacterial nanocomposites. The materials were challenged with models of Gram-negative and Gram-positive bacteria. For visible light, the average percentage killed (PK) was 94.6 for E. coli and 92.3 for S. aureus. For near-infrared light, PK for E. coli reported 52.8 and 99.2 for S. aureus. These results confirm the exciting potential of these nanocomposites to prevent the development of antibiotic resistance and also to open the door for further studies to optimize their composition in order to increase their bactericidal efficacy for biomedical applications.
Antibiotic resistance in bacteria is a major health concern. Antimicrobial Peptides (AMPs) are efficient in killing most microbes and yet the development of resistance to AMPs is rare. Although AMPs show promising antimicrobial activities, commercializing them as antibiotics is difficult as in vitro extraction and purification of AMPs is complicated and expensive. AMP mimicking antimicrobial polymers can overcome such problems while maintaining the necessary features of AMPs. Here, we have developed meth-acrylamide based polymers to mimic AMPs which possess high antimicrobial activities with low cytotoxicity. Bactericidal and scanning electron microscopy studies show that the synthesized polymers are effective against Gram-positive and Gram-negative bacteria. We find that these polymers are lethal to bacteria and at the same time, they are also non-cytotoxic to mammalian cells, thereby increasing the potential of these polymers to be used as antibiotics.
Grumezescu, Valentina, Negut, Irina, Cristescu, Rodica, Grumezescu, Alexandru Mihai, Holban, Alina Maria, Iordache, Florin, Chifiriuc, Mariana Carmen, Narayan, Roger J., and Chrisey, Douglas B. Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization. Retrieved from https://par.nsf.gov/biblio/10321203. Molecules 26.12 Web. doi:10.3390/molecules26123634.
Grumezescu, Valentina, Negut, Irina, Cristescu, Rodica, Grumezescu, Alexandru Mihai, Holban, Alina Maria, Iordache, Florin, Chifiriuc, Mariana Carmen, Narayan, Roger J., & Chrisey, Douglas B. Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization. Molecules, 26 (12). Retrieved from https://par.nsf.gov/biblio/10321203. https://doi.org/10.3390/molecules26123634
Grumezescu, Valentina, Negut, Irina, Cristescu, Rodica, Grumezescu, Alexandru Mihai, Holban, Alina Maria, Iordache, Florin, Chifiriuc, Mariana Carmen, Narayan, Roger J., and Chrisey, Douglas B.
"Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization". Molecules 26 (12). Country unknown/Code not available. https://doi.org/10.3390/molecules26123634.https://par.nsf.gov/biblio/10321203.
@article{osti_10321203,
place = {Country unknown/Code not available},
title = {Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization},
url = {https://par.nsf.gov/biblio/10321203},
DOI = {10.3390/molecules26123634},
abstractNote = {Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrrolidone/antibiotic/isoflavonoid thin films by the matrix-assisted pulsed laser evaporation (MAPLE) method as anti-adhesion barrier coatings, on biomedical surfaces for improved resistance to microbial colonization. The thin films were characterized by Fourier transform infrared spectroscopy, infrared microscopy, and scanning electron microscopy. In vitro biological assay tests were performed to evaluate the influence of the thin films on the development of biofilms formed by Gram-positive and Gram-negative bacterial strains. In vitro biocompatibility tests were assessed on human endothelial cells examined for up to five days of incubation, via qualitative and quantitative methods. The results of this study revealed that the laser-fabricated coatings are biocompatible and resistant to microbial colonization and biofilm formation, making them successful candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission.},
journal = {Molecules},
volume = {26},
number = {12},
author = {Grumezescu, Valentina and Negut, Irina and Cristescu, Rodica and Grumezescu, Alexandru Mihai and Holban, Alina Maria and Iordache, Florin and Chifiriuc, Mariana Carmen and Narayan, Roger J. and Chrisey, Douglas B.},
}
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