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1. Distal amyloid β-protein fragments template amyloid assembly: Distal Amyloid Β-Protein Fragments Template Amyloid Assembly

   https://doi.org/10.1002/pro.3375  

   Do, Thanh D. ; Sangwan, Smriti ; de Almeida, Natália E. C. ; Ilitchev, Alexandre I. ; Giammona, Maxwell ; Sawaya, Michael R. ; Buratto, Steven K. ; Eisenberg, David S. ; Bowers, Michael T. ( February 2018 , Protein Science)
2. Dual‐Functional, Multi‐Targeting GNNQQNY‐AIE Conjugates as Amyloid Probes and Amyloid Modulators via Amyloid Cross‐Seeding Principle

   https://doi.org/10.1002/adfm.202208022  

   Tang, Yijing ; Zhang, Dong ; Gong, Xiong ; Zheng, Jie ( September 2022 , Advanced Functional Materials)

   Amyloid protein aggregation is associated with many neurodegenerative diseases, including amyloid‐β (Aβ)in Alzheimer disease, human islet amyloid polypeptide (hIAPP) in type II diabetes, and human calcitonin (hCT) in medullary thyroid carcinoma. Significant efforts have been made to develop different diagnostic and prevention strategies for the early detection and intervention of these disease‐causative protein aggregates. However, conventional design wisdoms are mostly limited to the molecules with either single function (amyloid imaging or amyloid prevention) or single targeting protein (Aβ, hIAPP, or hCT). Here, a rational design strategy of an amyloid‐aggregation‐induced emission (AIE)‐active molecule is demonstrated by conjugating an amyloid fragment of GNNQQNY (G7) with an AIE fluorescent molecule of triphenylvinyl benzoic acid (namely, G7‐TBA), making G7‐TBA as multiple‐target, dual‐function, amyloid probes and amyloid modulators for detecting, monitoring, and altering amyloid aggregation of three different amyloid proteins (Aβ, hIAPP, and hCT). G7‐TBA probe shows conformationally specific binding affinities to amyloid aggregates, switching from an “off” state (low fluorescence) for amyloid monomers to an “on” state (high fluorescence) for β‐structure‐rich amyloid oligomers and fibrils in aqueous solution. Further surface immobilization of TBA probes on surface plasmon resonance surfaces allows to amplify detection sensitivity and binding affinity to amyloid aggregates formed at different aggregation stages. G7‐TBA as amyloid modulator enables acceleration of amyloid fibrillization and selectively protects cells from hIAPP‐induced toxicity. The distinct amyloid detection and modulation of G7‐TBA are essentially derived from the cross‐seeding between G7 and amyloid aggregation via β‐structure interaction, which by far exceed the binding affinity between commercial ThT and amyloid aggregates. Such design concepts of amyloid‐AIE conjugates can be further explored as multiple‐function and target probes and/or modulators for biomedical applications.

    

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3. Long-term, super-resolution imaging of amyloid structures using transient amyloid binding microscopy

   https://doi.org/10.1117/12.2507656  

   Ding, Tianben ; Spehar, Kevin ; Bieschke, Jan ; Lew, Matthew D. ( February 2019 , Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII)

   Amyloid fibrils and tangles are signatures of Alzheimer disease, but nanometer-sized aggregation intermediates are hypothesized to be the structures most toxic to neurons. The structures of these oligomers are too small to be resolved by conventional light microscopy. We have developed a simple and versatile method, called transient amyloid binding (TAB), to image amyloid structures with nanoscale resolution using amyloidophilic dyes, such as Thioflavin T, without the need for covalent labeling or immunostaining of the amyloid protein. Transient binding of ThT molecules to amyloid structures over time generates photon bursts that are used to localize single fluorophores with nanometer precision. Continuous replenishment of fluorophores from the surrounding solution minimizes photobleaching, allowing us to visualize a single amyloid structure for hours to days. We show that TAB microscopy can image both the oligomeric and fibrillar stages of amyloid-β aggregation. We also demonstrate that TAB microscopy can image the structural remodeling of amyloid fibrils by epi-gallocatechin gallate. Finally, we utilize TAB imaging to observe the non-linear growth of amyloid fibrils. 

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4. Star Polymers Reduce Islet Amyloid Polypeptide Toxicity via Accelerated Amyloid Aggregation

   https://doi.org/10.1021/acs.biomac.7b01301  

   Pilkington, Emily H. ; Lai, May ; Ge, Xinwei ; Stanley, William J. ; Wang, Bo ; Wang, Miaoyi ; Kakinen, Aleksandr ; Sani, Marc-Antonie ; Whittaker, Michael R. ; Gurzov, Esteban N. ; et al ( December 2017 , Biomacromolecules)
5. Protection against β-amyloid neurotoxicity by a non-toxic endogenous N-terminal β-amyloid fragment and its active hexapeptide core sequence

   https://doi.org/10.1111/jnc.14257  

   Forest, Kelly H. ; Alfulaij, Naghum ; Arora, Komal ; Taketa, Ruth ; Sherrin, Tessi ; Todorovic, Cedomir ; Lawrence, James L. ; Yoshikawa, Gene T. ; Ng, Ho-Leung ; Hruby, Victor J. ; et al ( January 2018 , Journal of Neurochemistry)