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Title: Fusion peptide from SARS-2 spike transforms into a wedge inserted in a bilayer leaflet, and thins the opposite leaflet
Activation of SARS-CoV-2 Spike deploys its fusion peptide to a membrane of the host cell to infect it. NMR in solution demonstrates that this fusion peptide transforms from intrinsic disorder in solution into a wedge-shaped structure inserted in bilayered micelles. According to NOEs and proximity to a nitroxide spin label deep in the membrane mimic, the globular fold of three helices contrasts the open, extended conformations observed in compact prefusion states. In the hydrophobic, narrow end of the wedge, helices 1 and 2 contact the fatty acyl chains of phospholipids. 50 of the resulting paramagnetic NMR relaxation enhancements and 6 lipid-protein NOEs provided ambiguous distances as collective variables (colvars) to bias and guide MD simulations. Simulations in NAMD using the CHARMM36 forcefield included colvars for 130 medium- and long-range NOEs to maintain the equilibrium structure. In the gently NMR-biased simulations, the fusion peptide maintained its insertion of helices 1 and 2 within a single leaflet while helix 3 remained exposed. A cation occasionally visited the anionic side chains in the loop joining helices 2 and 3 or at the N-terminal end of helix 1. The unoccupied leaflet is thinned and distorted opposite the fusion peptide.The thinning could be related to the fusion peptide promoting formation of the hemi-fusion intermediate in the process of viral-cell fusion. Supported by NSF Rapid award 2030473.  more » « less
Award ID(s):
2030473
NSF-PAR ID:
10331355
Author(s) / Creator(s):
Date Published:
Journal Name:
Biophysical journal
Volume:
121
Issue:
3
ISSN:
1542-0086
Page Range / eLocation ID:
74a
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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