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Title: Stochastic Hazard Analysis of Genetic Circuits in iBioSim and STAMINA
In synthetic biology, combinational circuits are used to program cells for various new applications like biosensors, drug delivery systems, and biofuels. Similar to asynchronous electronic circuits, some combinational genetic circuits may show unwanted switching variations (glitches) caused by multiple input changes. Depending on the biological circuit, glitches can cause irreversible effects and jeopardize the circuit’s functionality. This paper presents a stochastic analysis to predict glitch propensities for three implementations of a genetic circuit with known glitching behavior. The analysis uses STochastic Approximate Model-checker for INfinite-state Analysis (STAMINA), a tool for stochastic verification. The STAMINA results were validated by comparison to stochastic simulation in iBioSim resulting in further improvements of STAMINA. This paper demonstrates that stochastic verification can be utilized by genetic designers to evaluate design choices and input restrictions to achieve a desired reliability of operation.  more » « less
Award ID(s):
1856733 1856740
PAR ID:
10331952
Author(s) / Creator(s):
; ; ; ; ; ;
Date Published:
Journal Name:
ACS synthetic biology
Volume:
10
Issue:
10
ISSN:
2161-5063
Page Range / eLocation ID:
2532-2540
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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