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   https://doi.org/10.1021/acsomega.2c04358  

   Zhang, Jiahui ; Fakharzadeh, Ashkan ; Roland, Christopher ; Sagui, Celeste ( October 2022 , ACS Omega)
2. Efficient inhibition of RNA self-primed extension by addition of competing 3′-capture DNA-improved RNA synthesis by T7 RNA polymerase

   https://doi.org/10.1093/nar/gkz700  

   Gholamalipour, Yasaman ; Johnson, William C ; Martin, Craig T ( August 2019 , Nucleic Acids Research)

   Abstract In vitro synthesized RNA is used widely in studies of RNA biology, biotechnology and RNA therapeutics. However, in vitro synthesized RNA often contains impurities, such as RNAs with lengths shorter and longer than the expected runoff RNA. We have recently confirmed that longer RNA products are formed predominantly via cis self-primed extension, in which released runoff RNA folds back on itself to prime its own RNA-templated extension. In the current work, we demonstrate that addition of a DNA oligonucleotide (capture DNA) that is complementary to the 3′ end of the expected runoff RNA effectively prevents self-primed extension, even under conditions commonly used for high RNA yields. Moreover, the presence of this competing capture DNA during ‘high yield’ transcription, leads to an increase in the yield of expected runoff RNA by suppressing the formation of undesired longer RNA byproducts. 

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3. 3′ end additions by T7 RNA polymerase are RNA self-templated, distributive and diverse in character—RNA-Seq analyses

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4. A Novel Assay for RNA Polymerase I Transcription Elongation Sheds Light on the Evolutionary Divergence of Eukaryotic RNA Polymerases

   https://doi.org/10.1021/acs.biochem.8b01256  

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   https://doi.org/10.1186/s12864-020-6673-2  

   Scholes, Amanda N. ; Lewis, Jeffrey A. ( December 2020 , BMC Genomics)