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Chemical Triphosphorylation of Oligonucleotides

https://doi.org/10.3791/63877

Bare, Grant A. ; Horning, David P. ( January 2022 , Journal of Visualized Experiments)

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Cross-Chiral, RNA-Catalyzed Exponential Amplification of RNA

https://doi.org/10.1021/jacs.1c09233

Bare, Grant A. ; Joyce, Gerald F. ( November 2021 , Journal of the American Chemical Society)

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Assembly of long l -RNA by native RNA ligation

https://doi.org/10.1039/D1CC04296C

Yu, Chen-Hsu ; Kabza, Adam M. ; Sczepanski, Jonathan T. ( October 2021 , Chemical Communications)

Due to their intrinsic nuclease resistance, l -oligonucleotides are being increasingly utilized in the development of molecular tools and sensors. Yet, it remains challenging to synthesize long l -oligonucleotides, potential limiting future applications. Herein, we report straightforward and versitile approach to assemble long l -RNAs from two or more shorter fragments using T4 RNA ligase 1. We show that this approach is compatible with the assembly of several classes of functional l -RNA, which we highlight by generating a 124 nt l -RNA biosensor that functions in serum.
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