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Title: Open Droplet Microfluidics for Testing Multi-Drug Resistance and Antibiotic Resilience in Bacteria
New combinations of existing antibiotics are being investigated to combat bacterial resilience. This requires detection technologies with reasonable cost, accuracy, resolution, and throughput. Here, we present a multi -drug screening platform for bacterial cultures by combining droplet microfluidics, search algorithms, and imaging with a wide field of view. We remotely alter the chemical microenvironment around cells and test 12 combinations of resistant cell types and chemicals. Fluorescence intensity readouts allow us to infer bacterial resistance to specific antibiotics within 8 hours. The platform has potential to detect and identify parameters of bacterial resilience in cell cultures, biofilms, and microbial aggregates.  more » « less
Award ID(s):
1556370
NSF-PAR ID:
10334523
Author(s) / Creator(s):
; ; ;
Date Published:
Journal Name:
Transducers
Volume:
2021
Page Range / eLocation ID:
988 to 991
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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