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Title: Dynamic 3D On-Chip BBB Model Design, Development, and Applications in Neurological Diseases
The blood–brain barrier (BBB) is a vital structure for maintaining homeostasis between the blood and the brain in the central nervous system (CNS). Biomolecule exchange, ion balance, nutrition delivery, and toxic molecule prevention rely on the normal function of the BBB. The dysfunction and the dysregulation of the BBB leads to the progression of neurological disorders and neurodegeneration. Therefore, in vitro BBB models can facilitate the investigation for proper therapies. As the demand increases, it is urgent to develop a more efficient and more physiologically relevant BBB model. In this review, the development of the microfluidics platform for the applications in neuroscience is summarized. This article focuses on the characterizations of in vitro BBB models derived from human stem cells and discusses the development of various types of in vitro models. The microfluidics-based system and BBB-on-chip models should provide a better platform for high-throughput drug-screening and targeted delivery.  more » « less
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National Science Foundation
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    Basic Protocol 1: Isolation of primary mouse brain astrocytes and endothelial cells

    Basic Protocol 2: Isolation of primary mouse bone marrow–derived dendritic cells

    Support Protocol 1: Validation of dendritic cell purity by flow cytometry

    Basic Protocol 3: Isolation of primary mouse peripheral blood mononuclear cells

    Support Protocol 2: Isolation of primary mouse spleen cells

    Support Protocol 3: Purification and validation of CD4+ T cells from PBMCs and spleen cells

    Basic Protocol 4: Isolation of liver granuloma supernatant and determination of organ load

    Support Protocol 4: In vivo and in vitro infection with mycobacteria

    Basic Protocol 5: Assembly of the BBB co‐culture model

    Basic Protocol 6: Assembly of the combined in vitro granuloma and BBB model

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