skip to main content


Title: Global patterns in copepod thermal tolerance
Abstract Copepods are key components of aquatic habitats across the globe. Understanding how they respond to warming is important for predicting the effects of climate change on aquatic communities. Lethal thermal limits may play an important role in determining responses to warming. Thermal tolerance can vary over several different spatial and temporal scales, but we still lack a fundamental understanding of what drives the evolution of these patterns in copepods. In this Horizons piece, we provide a synthesis of global patterns in copepod thermal tolerance and potential acclimatory capacities. Copepod thermal tolerance increases with maximum annual temperature. We also find that the effects of phenotypic plasticity on thermal tolerance are negatively related to the magnitude of thermal tolerance, suggesting a potential trade-off between these traits. Our ability to fully describe these patterns is limited, however, by a lack of spatial, temporal and phylogenetic coverage in copepod thermal tolerance data. We indicate several priority areas for future work on copepod thermal tolerance, and accompanying suggestions regarding experimental design and methodology.  more » « less
Award ID(s):
1947965
NSF-PAR ID:
10344281
Author(s) / Creator(s):
;
Date Published:
Journal Name:
Journal of Plankton Research
Volume:
43
Issue:
4
ISSN:
0142-7873
Page Range / eLocation ID:
598 to 609
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. Abstract

    Differences in population vulnerability to warming are defined by spatial patterns in thermal adaptation. These patterns may be driven by natural selection over spatial environmental gradients, but can also be shaped by gene flow, especially in marine taxa with high dispersal potential. Understanding and predicting organismal responses to warming requires disentangling the opposing effects of selection and gene flow. We begin by documenting genetic divergence of thermal tolerance and developmental phenotypic plasticity. Ten populations of the widespread copepodAcartia tonsawere collected from sites across a large thermal gradient, ranging from the Florida Keys to Northern New Brunswick, Canada (spanning over 20° latitude). Thermal performance curves (TPCs) from common garden experiments revealed local adaptation at the sampling range extremes, with thermal tolerance increasing at low latitudes and decreasing at high latitudes. The opposite pattern was observed in phenotypic plasticity, which was strongest at high latitudes. No relationship was observed between phenotypic plasticity and environmental variables. Instead, the results are consistent with the hypothesis of a trade‐off between thermal tolerance and the strength of phenotypic plasticity. Over a large portion of the sampled range, however, we observed a remarkable lack of differentiation of TPCs. To examine whether this lack of divergence is the result of selection for a generalist performance curve or constraint by gene flow, we analyzed cytochrome oxidase I mtDNA sequences, which revealed four distinct genetic clades, abundant genetic diversity, and widely distributed haplotypes. Strong divergence in thermal performance within genetic clades, however, suggests that the pace of thermal adaptation can be relatively rapid. The combined insight from the laboratory physiological experiments and genetic data indicate that gene flow constrains differentiation of TPCs. This balance between gene flow and selection has implications for patterns of vulnerability to warming. Taking both genetic differentiation and phenotypic plasticity into account, our results suggest that local adaptation does not increase vulnerability to warming, and that low‐latitude populations in general may be more vulnerable to predicted temperature change over the next century.

     
    more » « less
  2. Abstract

    Organismal thermal limits affect a wide range of biogeographical and ecological processes. Copepods are some of the most abundant animals on the planet and play key roles in aquatic habitats. Despite their abundance and ecological importance, there is limited data on the factors that affect copepod thermal limits, impeding our ability to predict how aquatic ecosystems will be affected by anthropogenic climate change. In a warming ocean, one factor that may have particularly important effects on thermal limits is the availability of food. A recently proposed feedback loop known as “metabolic meltdown” suggests that starvation and exposure to high temperatures interact to drastically reduce organismal thermal limits, increasing vulnerability to warming. To investigate one component of this feedback loop, we examined how starvation affects thermal limits (critical thermal maxima: CTmax) ofAcartia tonsa, a widespread estuarine copepod. We found that there was no effect of short‐duration exposure to starvation (up to 2 days). However, after 3 days, there was a significant decrease in the CTmaxof starved copepods relative to the fed controls. Our results provide empirical evidence that extended periods of starvation reduce thermal limits, potentially initiating “metabolic meltdown” in this key species of coastal copepod. This suggests that changes in food availability may increase the vulnerability of copepods to increasing temperatures, amplifying the effects of climate change on coastal systems.

     
    more » « less
  3. Synopsis

    Accurate forecasting of organismal responses to climate change requires a deep mechanistic understanding of how physiology responds to present-day variation in the physical environment. However, the road to physiological enlightenment is fraught with complications: predictable environmental fluctuations of any single factor are often accompanied by substantial stochastic variation and rare extreme events, and several factors may interact to affect physiology. Lacking sufficient knowledge of temporal patterns of co-variation in multiple environmental stressors, biologists struggle to design and implement realistic and relevant laboratory experiments. In this study, we directly address these issues, using measurements of the thermal tolerance of freshly collected animals and long-term field records of environmental conditions to explore how the splash-pool copepod Tigriopus californicus adjusts its physiology as its environment changes. Salinity and daily maximum temperature—two dominant environmental stressors experienced by T. californicus—are extraordinarily variable and unpredictable more than 2–3 days in advance. However, they substantially co-vary such that when temperature is high salinity is also likely to be high. Copepods appear to take advantage of this correlation: median lethal temperature of field-collected copepods increases by 7.5°C over a roughly 120 parts-per-thousand range of ambient salinity. Complementary laboratory experiments show that exposure to a single sublethal thermal event or to an abrupt shift in salinity also elicits rapid augmentation of heat tolerance via physiological plasticity, although the effect of salinity dwarfs that of temperature. These results suggest that T. californicus’s physiology keeps pace with the rapid, unpredictable fluctuations of its hypervariable physical environment by responding to the cues provided by recent sublethal stress and, more importantly, by leveraging the mechanistic cross-talk between responses to salinity and heat stress.

     
    more » « less
  4. Abstract

    Climate change is resulting in increasing ocean temperatures and salinity variability, particularly in estuarine environments. Tolerance of temperature and salinity change interact and thus may impact organismal resilience. Populations can respond to multiple stressors in the short‐term (i.e., plasticity) or over longer timescales (i.e., adaptation). However, little is known about the short‐ or long‐term effects of elevated temperature on the tolerance of acute temperature and salinity changes. Here, we characterized the response of the near‐shore and estuarine copepod,Acartia tonsa, to temperature and salinity stress. Copepods originated from one of two sets of replicated >40 generation‐old temperature‐adapted lines: ambient (AM, 18°C) and ocean warming (OW, 22°C). Copepods from these lines were subjected to one and three generations at the reciprocal temperature. Copepods from all treatments were then assessed for differences in acute temperature and salinity tolerance. Development (one generation), three generations, and >40 generations of warming increased thermal tolerance compared to Ambient conditions, with development in OW resulting in equal thermal tolerance to three and >40 generations of OW. Strikingly, developmental OW and >40 generations of OW had no effect on low salinity tolerance relative to ambient. By contrast, when environmental salinity was reduced first, copepods had lower thermal tolerances. These results highlight the critical role for plasticity in the copepod climate response and suggest that salinity variability may reduce copepod tolerance to subsequent warming.

     
    more » « less
  5. Kumar, Ram (Ed.)
    Short-term, acute warming events are increasing in frequency across the world’s oceans. For short-lived species like most copepods, these extreme events can occur over both within- and between-generational time scales. Yet, it is unclear whether exposure to acute warming during early life stages of copepods can cause lingering effects on metabolism through development, even after the event has ended. These lingering effects would reduce the amount of energy devoted to growth and affect copepod population dynamics. We exposed nauplii of an ecologically important coastal species, Acartia tonsa , to a 24-hour warming event (control: 18°C; treatment: 28°C), and then tracked individual respiration rate, body length, and stage duration through development. As expected, we observed a decrease in mass-specific respiration rates as individuals developed. However, exposure to acute warming had no effect on the ontogenetic patterns in per-capita or mass-specific respiration rates, body length, or development time. The lack of these carryover effects through ontogeny suggests within-generational resilience to acute warming in this copepod species. 
    more » « less