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Title: Facile and Sensitive Detection of Nitrogen-Containing Organic Bases with Near Infrared C-Dots Derived Assays
In this article, we have designed both colorimetric (including solution and test paper type) and spectral sensors (including UV-vis and PL type) for the quick and sensitive detection of general nitrogen-containing organic bases (NCOBs); the limit of detection could reach as low as 0.50 nM. NCOBs included 11 examples, covering aliphatic and aromatic amines, five- and six-membered heterocyclics, fused-ring heterocyclics, amino acids, and antibiotics. Furthermore, the assays demonstrated high reliability in sensing NCOBs and excellent ability to distinguish NCOBs from oxygen and sulfur containing organics. The assays developed could find important applications for the detection of NCOBs in the fields of biomedicine, chemistry, and agriculture.  more » « less
Award ID(s):
2041413
NSF-PAR ID:
10350450
Author(s) / Creator(s):
; ; ; ; ; ; ;
Date Published:
Journal Name:
Nanomaterials
Volume:
11
Issue:
10
ISSN:
2079-4991
Page Range / eLocation ID:
2607
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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    Alternate Protocol 1: Preparation of crude extracts from Jack bean meal

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    Alternate Protocol 3: Preparation ofEscherichia colicell lysates containing human galectin‐3

    Alternate Protocol 4: Preparation of crude extracts from chicken egg whites (source of ovalbumin)

    Basic Protocol 2: Preparation of 2% (v/v) red blood cell suspension

    Basic Protocol 3: Detection of lectin activity of the crude extracts

    Basic Protocol 4: Identification of multivalent inhibitors as target capturing agents by hemagglutination inhibition assays

    Basic Protocol 5: Testing the capturing abilities of target capturing agents by precipitation/turbidity assays

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    Basic Protocol 7: Releasing the captured targets (lectins and glycoproteins) by dissolving the complex

    Basic Protocol 8: Separation of the targets (lectins and glycoproteins) from their respective target capturing agents

    Basic Protocol 9: Verification of the purity of the isolated targets (lectins or glycoproteins)

     
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