skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.


Title: An optogenetics device with smartphone video capture to introduce neurotechnology and systems neuroscience to high school students
Although neurotechnology careers are on the rise, and neuroscience curriculums have significantly grown at the undergraduate and graduate levels, increasing neuroscience and neurotechnology exposure in high school curricula has been an ongoing challenge. This is due, in part, to difficulties in converting cutting-edge neuroscience research into hands-on activities that are accessible for high school students and affordable for high school educators. Here, we describe and characterize a low-cost, easy-to-construct device to enable students to record rapid Drosophila melanogaster (fruit fly) behaviors during optogenetics experiments. The device is generated from inexpensive Arduino kits and utilizes a smartphone for video capture, making it easy to adopt in a standard biology laboratory. We validate this device is capable of replicating optogenetics experiments performed with more sophisticated setups at leading universities and institutes. We incorporate the device into a high school neuroengineering summer workshop. We find student participation in the workshop significantly enhances their understanding of key neuroscience and neurotechnology concepts, demonstrating how this device can be utilized in high school settings and undergraduate research laboratories seeking low-cost alternatives.  more » « less
Award ID(s):
1921065 1747506
PAR ID:
10352330
Author(s) / Creator(s):
; ; ; ; ;
Editor(s):
Louis, Matthieu
Date Published:
Journal Name:
PLOS ONE
Volume:
17
Issue:
5
ISSN:
1932-6203
Page Range / eLocation ID:
e0267834
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. With grant support from the Research Experience for Undergraduates (REU) program funded by the National Science Foundation (NSF) and the Awards to Stimulate and Support Undergraduate Research Experiences (ASSURE) program funded by the Department of Defense (DoD) Air Force Office of Scientific Research (AFOSR), we established a program intended to increase the number of underrepresented racial and ethnic minority (URM) and first-generation undergraduate students successfully applying to neuroscience and other STEM-related graduate programs. The Neuroscience Techniques and Research Training (NeuroSTART) Program aimed to increase the number of undergraduate students from the Memphis area involved in behavioral neuroscience research. In this two-semester program, students completed an empirical research project in a neuroscience lab, received individual mentoring from neuroscience faculty, became part of a STEM network, presented at research conferences, and attended specialized professional development seminars. In two cohorts of 15 students, 4 are PhD students in neuroscience-related programs or in medical school (27%), 4 are employed in neuroscience-related research facilities (27%), 3 are employed as clinical assistants (20%), and 1 is employed in the IT field (7%). The remaining three recently graduated and are planning a gap year prior to applying for admission to grad/medical school. The Memphis NeuroSTART program has provided valuable training to participants, making them competitive applicants for jobs in the health sciences and for admittance into graduate neuroscience programs. By providing this training to first-generation and URM students, the broader impact of this program was an increase in the diversity of the health sciences workforce, particularly those specializing in neuroscience-related research and treatment. 
    more » « less
  2. Compared to traditional teaching laboratory activities, course-based undergraduate research experiences (CUREs) can increase student engagement and confidence, improve scientific literacy, enhance critical thinking, and promote accessibility in STEM. Here we describe a versatile CURE for an upper-level Neurobiology course that incorporates genetic, molecular, cellular, and behavioral experiments into a semester-long investigation to identify genes important for glutamate synapse formation or function in C. elegans. Following introduction to the CURE approach and basic C. elegans techniques, students construct their own low-cost optogenetics rigs, which we describe in detail here, to activate a mechanosensory escape reflex via photostimulation. They then perform a small-scale RNAi screen with this light-activated behavioral readout. Once a gene of interest is identified, students submit a proposal to investigate the role of this gene in nervous system function and spend the rest of the semester carrying out follow-up experiments using mutant strains. We also describe ways in which this CURE can be modified depending on the pedagogical objectives, availability of materials, or research interests of the instructor. Participating in this lab significantly enhanced students' abilities to see themselves as STEM professionals and prompted students to report substantial gains in skills critical for entry into and success in graduate and medical schools. In addition to the benefits CUREs provide to students, faculty benefit from the generation of preliminary data and training of students for potential independent research projects. 
    more » « less
  3. This outreach undergraduate research project presents a low-cost method to distinguish the quality of different olive oils. The proposed method is based on an indirect measurement of the chlorophyll molecules present when a green laser diode illuminates the oil sample. Oil blends can be classified into five classes (no olive oil, light olive oil, medium olive oil, olive oil, and extra virgin olive oil) by quantifying the ratio of the red channel versus the green channel along the laser illumination path from a color image. After labeling each oil blend, a convolutional neural network has been implemented and trained to automatically classify oil blends from a color image. The trained convolutional neural network has an accuracy of 90% in identifying and categorizing oil blends. This undergraduate research project introduces students to an interdisciplinary application requiring the combination of optical spectroscopy (i.e., multicolor imaging), image processing, and machine learning. In addition, due to the simplicity of the optical apparatus and computational analysis, high school students could implement and validate their own costeffective oil-quality classification device. 
    more » « less
  4. Historically, there has been a challenge of retaining and graduating students as STEM majors. At CSU Dominguez Hills, a regional, urban, Hispanic-Serving and Minority-Serving Institution with a large number of first-generation college students that receive Pell grants, student persistence and retention is of particular concern.The purpose of this study is to study the efficacy of combining Course-based Undergraduate Research Experiences (CUREs), High-Impact Educational Practices (HIPs), Design Thinking (DT) training and Strengths-based coaching into a First Year Seminar (FYS) course. A diverse group of first-year students from both STEM and non-STEM majors enrolled in a Neuroscience of Hedonism course where they participated in a variety of activities to 1) promote learning of basic neuroscience concepts, 2) conduct a research study using low-cost electrophysiology tools, and 3) support personal and professional development. In addition to studying long-term effects like student retention and persistence rates, we also measure recruitment of non-STEM majors to STEM majors, science identity/literacy, self-efficacy and a variety of career-related attitudes. This pilot study will provide a framework by which STEM departments can create a survey course to recruit incoming freshmen and encourage retention and persistence in STEM majors and subsequent careers. 
    more » « less
  5. Haptic technology has the potential to expand and transform the ways that students can experience a variety of science, technology, engineering, and math (STEM) topics. Designing kinesthetic haptic devices for educational applications is challenging because of the competing objectives of using low-cost components, making the device robust enough to be handled by students, and the desire to render high fidelity haptic virtual environments. In this paper, we present the evolution of a device called "Hapkit": a low cost, one-degree-of-freedom haptic kit that can be assembled by students. From 2013-2015, different versions of Hapkit were used in courses as a tool to teach haptics, physics, and control. These include a Massive Open Online Course (MOOC), two undergraduate courses, a graduate course, and a middle school class. Based on our experience using Hapkit in these educational environments, we evolved the design in terms of its structural materials, drive mechanism, and mechatronic components. Our latest design, Hapkit 3.0, includes several features that allow students to manufacture and assemble a robust and high-fidelity haptic device. First, it uses 3-D printed plastic structural material, which allows the design to be built and customized using readily available tools. Second, the design takes into account the limitations of 3-D printing, such as warping during printing and poor tolerances. This is achieved at a materials cost of approximately US $50, which makes it feasible for distribution in classroom and online education settings. The open source design is available at http://hapkit.stanford.edu. 
    more » « less