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Biofilm formation is a significant problem in America, accounting for 17 million infections, and causing 550,000 deaths annually. An understanding of factors that contribute to strong biofilm surface adhesion at implant interfaces can guide the development of surfaces that prevent deleterious biofilms and promote osseointegration. The aim of this research is to develop a metric that quantifies the adhesion strength differential between a bacterial biofilm and an osteoblast-like cell monolayer to a medical implant-simulant surface. This metric will be used to quantify the biocompatible effect of implant surfaces on bacterial and cell adhesion. The laser spallation technique employs a high-amplitude short-duration stress wave to initiate spallation of biological films. Attenuation of laser energy results in failure statistics across increasing fluence values, which are calibrated via interferometry to obtain interface stress values. Several metrology challenges were overcome including how membrane tension may influence laser spallation testing and how to determine stress wave characteristics when surface roughness precludes in situ displacement measurements via interferometry. Experiments relating loading region within biofilm to centroid of biofilm revealed that location played no role in failure rate. A reflective panel was implemented to measure stress wave characteristics on smooth and rough titanium, which showed no difference in peak compressive wave amplitude. After overcoming these metrology challenges, the adhesion strength of Streptococcus mutans biofilms and MG 63 monolayers on smooth and rough titanium substrates is measured. An Adhesion Index is developed by obtaining the ratio of cell adhesion to biofilm adhesion. This nondimensionalized parameter represents the effect of surface modifications on increases or decreases in biocompatibility. An increase in Adhesion Index value is calculated for roughened titanium compared to smooth titanium. The increase in Adhesion Index values indicates that the increase in surface roughness has a more positive biological response from MG 63 than does S. mutans. In this work further experiments quantifying impact of various surface coating including blood plasma, and adhesion proteins found within the extracellular matrix to expand the Adhesion Index.
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Data: Sucrose-mediated formation and adhesion strength of Streptococcus mutans biofilms on titanium
Bacterial biofilms associated with implants remain a significant source of infections in dental, implant, and other healthcare industries due to challenges in biofilm removal. Biofilms consist of bacterial cells surrounded by a matrix of extracellular polymeric substance (EPS), which protects the colony from many countermeasures, including antibiotic treatments. Biofilm EPS composition is also affected by environmental factors. In the oral cavity, the presence of sucrose affects the growth of Streptococcus mutans that produce acids, eroding enamel and forming dental caries. Biofilm formation on dental implants commonly leads to severe infections and failure of the implant. This work determines the effect of sucrose concentration on biofilm EPS formation and adhesion of Streptococcus mutans, a common oral colonizer. Bacterial biofilms are grown with varying concentrations of sucrose on titanium substrates simulating dental implant material. Strategies for measuring adhesion for films such as peel tests are inadequate for biofilms, which have low cohesive strength and will fall apart when tensile loading is applied directly. The laser spallation technique is used to apply a stress wave loading to the biofilm, causing the biofilm to delaminate at a critical tensile stress threshold. Biofilm formation and EPS structures are visualized at high magnification with scanning electron microscopy (SEM). Sucrose enhanced the EPS production of S. mutans biofilms and increased the adhesion strength to titanium, the most prevalent dental implant material. However, there exists a wide range of sucrose concentrations that are conducive for robust formation and adhesion of S. mutans biofilms on implant surfaces.
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- Award ID(s):
- 2045853
- PAR ID:
- 10353241
- Publisher / Repository:
- Materials Data Facility
- Date Published:
- Subject(s) / Keyword(s):
- biomaterials biofilms laser spallation scanning electron microscopy adhesion dental implants streptococcus mutans sucrose titanium experiment
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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