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Title: Influence of Temperature and CO 2 On Plasma‐membrane Permeability to CO 2 and HCO 3 − in the Marine Haptophytes Emiliania huxleyi and Calcidiscus leptoporus (Prymnesiophyceae)

Membrane permeabilities to CO2and HCO3constrain the function of CO2concentrating mechanisms that algae use to supply inorganic carbon for photosynthesis. In diatoms and green algae, plasma membranes are moderately to highly permeable to CO2but effectively impermeable to HCO3. Here, CO2and HCO3membrane permeabilities were measured using an18O‐exchange technique on two species of haptophyte algae,Emiliania huxleyiandCalcidiscus leptoporus, which showed that the plasma membranes of these species are also highly permeable to CO2(0.006–0.02 cm · s−1) but minimally permeable to HCO3. Increased temperature and CO2generally increased CO2membrane permeabilities in both species, possibly due to changes in lipid composition or CO2channel proteins. Changes in CO2membrane permeabilities showed no association with the density of calcium carbonate coccoliths surrounding the cell, which could potentially impede passage of compounds. Haptophyte plasma‐membrane permeabilities to CO2were somewhat lower than those of diatoms but generally higher than membrane permeabilities of green algae. One caveat of these measurements is that the model used to interpret18O‐exchange data assumes that carbonic anhydrase, which catalyzes18O‐exchange, is homogeneously distributed in the cell. The implications of this assumption were tested using a two‐compartment model with an inhomogeneous distribution of carbonic anhydrase to simulate18O‐exchange data and then inferring plasma‐membrane CO2permeabilities from the simulated data. This analysis showed that the inferred plasma‐membrane CO2permeabilities are minimal estimates but should be quite accurate under most conditions.

 
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Award ID(s):
1744760
NSF-PAR ID:
10361770
Author(s) / Creator(s):
 ;  ;  ;  ;
Publisher / Repository:
Wiley-Blackwell
Date Published:
Journal Name:
Journal of Phycology
Volume:
56
Issue:
5
ISSN:
0022-3646
Page Range / eLocation ID:
p. 1283-1294
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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"Influence of Temperature and CO 2 On Plasma‐membrane Permeability to CO 2 and HCO 3 − in the Marine Haptophytes Emiliania huxleyi and Calcidiscus leptoporus (Prymnesiophyceae)". <em>Journal of Phycology</em> 56 (5). Country unknown/Code not available: Wiley-Blackwell. <a href="https://doi.org/10.1111/jpy.13017">https://doi.org/10.1111/jpy.13017.</a> <a href="https://par.nsf.gov/biblio/10361770">https://par.nsf.gov/biblio/10361770</a>. </div> <div class="modal-footer"> <button class="btn btn-sm btn-default" data-dismiss="modal" aria-hidden="true">Close</button> </div> </div> </div> </div></li> <li class="links-format"><a href="#cite-bib" data-toggle="modal">BibTeX</a> <div id="cite-bib" class="modal" tabindex="-1" role="dialog" aria-labelledby="cite-bib_label" aria-hidden="true"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal" aria-hidden="true">×</button> <strong id="cite-bib_label">Cite: BibTeX Format</strong> </div> <div class="modal-body"> @article{osti_10361770,<br/> place = {Country unknown/Code not available}, title = {Influence of Temperature and CO 2 On Plasma‐membrane Permeability to CO 2 and HCO 3 − in the Marine Haptophytes Emiliania huxleyi and Calcidiscus leptoporus (Prymnesiophyceae)}, url = {https://par.nsf.gov/biblio/10361770}, DOI = {10.1111/jpy.13017}, abstractNote = {Membrane permeabilities to CO2and HCO3−constrain the function of CO2concentrating mechanisms that algae use to supply inorganic carbon for photosynthesis. In diatoms and green algae, plasma membranes are moderately to highly permeable to CO2but effectively impermeable to HCO3−. Here, CO2and HCO3−membrane permeabilities were measured using an18O‐exchange technique on two species of haptophyte algae,Emiliania huxleyiandCalcidiscus leptoporus, which showed that the plasma membranes of these species are also highly permeable to CO2(0.006–0.02 cm · s−1) but minimally permeable to HCO3−. Increased temperature and CO2generally increased CO2membrane permeabilities in both species, possibly due to changes in lipid composition or CO2channel proteins. Changes in CO2membrane permeabilities showed no association with the density of calcium carbonate coccoliths surrounding the cell, which could potentially impede passage of compounds. Haptophyte plasma‐membrane permeabilities to CO2were somewhat lower than those of diatoms but generally higher than membrane permeabilities of green algae. One caveat of these measurements is that the model used to interpret18O‐exchange data assumes that carbonic anhydrase, which catalyzes18O‐exchange, is homogeneously distributed in the cell. The implications of this assumption were tested using a two‐compartment model with an inhomogeneous distribution of carbonic anhydrase to simulate18O‐exchange data and then inferring plasma‐membrane CO2permeabilities from the simulated data. This analysis showed that the inferred plasma‐membrane CO2permeabilities are minimal estimates but should be quite accurate under most conditions.}, journal = {Journal of Phycology}, volume = {56}, number = {5}, publisher = {Wiley-Blackwell}, author = {Blanco‐Ameijeiras, Sonia and Stoll, Heather M. and Zhang, Hongrui and Hopkinson, Brian M. and Raven, ed., J.}, }</div> <div class="modal-footer"> <button class="btn btn-sm btn-default" data-dismiss="modal" aria-hidden="true">Close</button> </div> </div> </div> </div></li> <li class="divider"></li> </ul> <ul class="nav nav-list" style="font-size: 14px; font-family: Arial Regular;"> <li class="nav-header header-format">Export Metadata</li> <li class="links-format"><a href="https://par.nsf.gov/endnote?osti_id=10361770">EndNote</a></li> <li class="links-format"><a href="https://par.nsf.gov/export/format:excel/osti-id:10361770">Excel</a></li> <li class="links-format"><a href="https://par.nsf.gov/export/format:csv/osti-id:10361770">CSV</a></li> <li class="links-format"><a href="https://par.nsf.gov/export/format:xml/osti-id:10361770">XML</a></li> <li class="divider"></li> </ul> <ul class="nav nav-list" style="font-size: 14px; 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