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Title: Isolation of Tobacco Mosaic Virus‐Binding Peptides for Biotechnology Applications
Abstract Tobacco mosaic virus (TMV) was the first virus to be discovered and it is now widely used as a tool for biological research and biotechnology applications. TMV particles can be decorated with functional molecules by genetic engineering or bioconjugation. However, this can destabilize the nanoparticles, and/or multiple rounds of modification may be necessary, reducing product yields and preventing the display of certain cargo molecules. To overcome these challenges, we used phage display technology and biopanning to isolate a TMV‐binding peptide (TBPT25) with strong binding properties (IC50=0.73 μM, KD=0.16 μM), allowing the display of model cargos via a single mixing step. The TMV‐binding peptide is specific for TMV but does not recognize free coat proteins and can therefore be used to decorate intact TMV or detect intact TMV particles in crude plant sap.  more » « less
Award ID(s):
2011924
PAR ID:
10370046
Author(s) / Creator(s):
 ;  
Publisher / Repository:
Wiley Blackwell (John Wiley & Sons)
Date Published:
Journal Name:
ChemBioChem
Volume:
23
Issue:
11
ISSN:
1439-4227
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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