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Abstract Quantification of genital variation in males and females can inform our understanding of likely copulatory interactions and evolution of genital diversity. However, no studies have quantified genital shape variation within a single snake species or examined the shape and size of both the vaginal pouch and hemipenes. Here, we examine the shape and size of the genitalia of female and male diamondback water snakes, Nerodia rhombifer, using a three-dimensional automated landmark geometric morphometric approach on models of the lumen of the vaginal pouch and inflated hemipenes, applying these techniques for the first time to the genital shape of vertebrates. Vaginal pouch shape is significantly associated with body size and reproductive status. As females grow larger and become reproductive, the vaginal pouch enlarges, widens and becomes more bifurcated. In reproductive males, the shape of the hemipenes is also significantly associated with body size. As males grow larger, the hemipenes enlarge and widen; their bifurcation becomes more defined and the spines at the base become more prominent. Vaginal pouch and hemipenial centroid size are isometric with respect to body length. The centroid sizes of the hemipenes and vaginal pouch are not significantly different from one another, hence the genitalia match in size. Reproductive females and males covary in the degree of bifurcation and size of their genitalia. We demonstrate the utility of three-dimensional analysis in studies of the shape of soft tissues and advocate its use in future studies of genitalia.
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Evolution and Morphology of Genitalia in Female Amniotes
Abstract Despite their evolutionary and biomedical importance, studies of the morphology and function of female genitalia have continued to lag behind those of male genitalia. While studying female genitalia can be difficult because of their soft, deformable and internal nature, recent advances in imaging, geometric analyses of shape and mechanical testing have been made, allowing for a much greater understanding of the incredible diversity of form and function of female genitalia. Here, we summarize some of these methods, as well as discuss some big questions in the field that are beginning to be examined now, and will continue to benefit from further work, especially a comparative approach. Topics of further research include examination of the morphology of female genitalia in situ, in-depth anatomical work in many more species, studies of the interplay between natural and sexual selection in influencing features of vaginal morphology, how these diverse functions influence the mechanical properties of tissues, and studies of clitoris morphology and function across amniotes. Many other research topics related to female genitalia remain largely unexplored, and we hope that the papers in this issue will continue to inspire further research on female genitalia.
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- Award ID(s):
- 2042260
- PAR ID:
- 10371812
- Publisher / Repository:
- Oxford University Press
- Date Published:
- Journal Name:
- Integrative And Comparative Biology
- Volume:
- 62
- Issue:
- 3
- ISSN:
- 1540-7063
- Page Range / eLocation ID:
- p. 521-532
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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