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Title: Rational design for high bioorthogonal fluorogenicity of tetrazine‐encoded green fluorescent proteins
Abstract Key points

Ultrafast spectroscopy reveals FRET in action and inhomogeneous populations with different transition dipole moment alignments.

Rational protein design of two new superfolder GFP mutants with record‐high fluorogenicity.

Bioimaging application of the designed bioorthogonal protein mutant in live eukaryotic cells.

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Award ID(s):
2003550 1817949 2054824
Author(s) / Creator(s):
 ;  ;  ;  ;  ;  
Publisher / Repository:
Wiley Blackwell (John Wiley & Sons)
Date Published:
Journal Name:
Natural Sciences
Medium: X
Sponsoring Org:
National Science Foundation
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