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Title: M-TUBE enables large-volume bacterial gene delivery using a high-throughput microfluidic electroporation platform
Conventional cuvette-based and microfluidics-based electroporation approaches for bacterial gene delivery have distinct advantages, but they are typically limited to relatively small sample volumes, reducing their utility for applications requiring high throughput such as the generation of mutant libraries. Here, we present a scalable, large-scale bacterial gene delivery approach enabled by a disposable, user-friendly microfluidic electroporation device requiring minimal device fabrication and straightforward operation. We demonstrate that the proposed device can outperform conventional cuvettes in a range of situations, including across Escherichia coli strains with a range of electroporation efficiencies, and we use its large-volume bacterial electroporation capability to generate a library of transposon mutants in the anaerobic gut commensal Bifidobacterium longum .  more » « less
Award ID(s):
2125383
PAR ID:
10376715
Author(s) / Creator(s):
; ; ; ; ;
Editor(s):
Waldor, Matthew K.
Date Published:
Journal Name:
PLOS Biology
Volume:
20
Issue:
9
ISSN:
1545-7885
Page Range / eLocation ID:
e3001727
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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