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Title: LimitedMechanisticLinkBetweentheMonodEquationand MethanogenGrowth:aPerspectivefromMetabolicModeling
The Monodequationhasbeenwidelyappliedasthegeneralratelaw of microbialgrowth,butitsapplicationsarenotalwayssuccessful.Bydrawingon the frameworksofkineticandstoichiometricmetabolicmodelsandmetaboliccon- trol analysis,themodelingreportedheresimulatedthegrowthkineticsofametha- nogenic microorganismandillustratedthatdifferentenzymesandmetabolitescon- trol growthratetovariousextentsandthattheircontrolspeakateitherverylow, intermediate, orveryhighsubstrateconcentrations.Incomparison,withasingle term andtwoparameters,theMonodequationonlyapproximatelyaccountsforthe controls ofrate-determiningenzymesandmetabolitesatveryhighandverylow substrate concentrations,butneglectstheenzymesandmetaboliteswhosecontrols are mostnotableatintermediateconcentrations.These findings supportalimited link betweentheMonodequationandmethanogengrowth,andunifythecompet- ing viewsregardingenzymerolesinshapinggrowthkinetics.Theresultsalsopre- clude amechanisticderivationoftheMonodequationfrommethanogenmetabolic networks andhighlightafundamentalchallengeinmicrobiology:single-termexpres- sions maynotbesufficient foraccuratepredictionofmicrobialgrowth.  more » « less
Award ID(s):
1753436
PAR ID:
10376740
Author(s) / Creator(s):
; ; ;
Editor(s):
Jin, Q; Wu, Q; Shapiro, B; McKernan, S.
Date Published:
Journal Name:
Microbiology spectrum
Volume:
10
Issue:
2
ISSN:
2165-0497
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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