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1. Thermophilic Anaerobic Digestion: Enhanced and Sustainable Methane Production from Co-Digestion of Food and Lignocellulosic Wastes

   https://doi.org/10.3390/en11082058  

   David, Aditi ; Govil, Tanvi ; Tripathi, Abhilash ; McGeary, Julie ; Farrar, Kylie ; Sani, Rajesh ( August 2018 , Energies)

   This article aims to study the codigestion of food waste (FW) and three different lignocellulosic wastes (LW) (Corn stover (CS), Prairie cordgrass (PCG), and Unbleached paper (UBP)) for thermophilic anaerobic digestion to overcome the limitations of digesting food waste alone (volatile fatty acids accumulation and low C:N ratio). Using an enriched thermophilic methanogenic consortium, all the food and lignocellulosic waste mixtures showed positive synergistic effects of codigestion. After 30 days of incubation at 60 °C (100 rpm), the highest methane yield of 305.45 L·kg−1 volatile solids (VS) was achieved with a combination of FW-PCG-CS followed by 279.31 L·kg−1 VS with a mixture of FW-PCG. The corresponding volatile solids reduction for these two co-digestion mixtures was 68% and 58%, respectively. This study demonstrated a reduced hydraulic retention time for methane production using FW and LW. 

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2. Energy valorization of cow manure by hydrothermal carbonization and anaerobic digestion

   https://doi.org/10.1016/j.renene.2020.07.003  

   Marin-Batista, J.D. ; Villamil, J.A. ; Qaramaleki, S.V. ; Coronella, C.J. ; Mohedano, A.F. ; Rubia, M.A. de ( November 2020 , Renewable Energy)

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3. Optimizing anaerobic co-digestion of goat manure and cotton gin trash using biochemical methane potential (BMP) test and mathematical modeling

   https://doi.org/10.1007/s42452-021-04706-1  

   Kaur, Harjinder ; Kommalapati, Raghava R. ( August 2021 , SN Applied Sciences)

   null (Ed.)

   Abstract Anaerobic co-digestion is widely adopted to enhance process efficacy by balancing the C/N ratio of the feedstock while converting organic wastes to biomethane. Goat manure (GM) and cotton gin trash (CGT) were anaerobically co-digested in triplicate batch bioreactors. The process was optimized and evaluated utilizing mathematical equations. The liquid fraction of the digestate was analyzed for nitrate and phosphate. The co-digestions with 10 and 20% CGT having the C/N ratios of 17.7 and 19.8 yielded the highest and statistically similar 261.4 ± 4.8 and 262.6 ± 4.2 mL/g vs biomethane, respectively. The biodegradability (BD) of GM and CGT was 94.5 ± 2.7 and 37.6 ± 0.8%, respectively. The BD decreased proportionally with an increase in CGT percentage. The co-digestion having 10% CGT yielded 80–90% of biomethane in 26–39 d. The modified Gompertz model-predicted and experimental biomethane values were similar. The highest synergistic effect index of 15.6 ± 4.7% was observed in GM/CGT; 30:70 co-digestion. The concentration of nitrate and phosphate was lower in the liquid fraction of digestate than the feedstocks, indicating that these nutrients stay in the solid fraction. The results provide important insights in agro-waste management, further studies determining the effects of effluent application on plants need to be conducted. 

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4. Adsorption or direct interspecies electron transfer? A comprehensive investigation of the role of biochar in anaerobic digestion of hydrothermal liquefaction aqueous phase

   https://doi.org/10.1016/j.cej.2022.135078  

   Wang, Zixin ; Zhang, Cheng ; Watson, Jamison ; Sharma, Brajendra K. ; Si, Buchun ; Zhang, Yuanhui ( May 2022 , Chemical Engineering Journal)
5. Optimization of Mechanical Tissue Dissociation Using an Integrated Microfluidic Device for Improved Generation of Single Cells Following Digestion

   https://doi.org/10.3389/fbioe.2022.841046  

   Aliaghaei, Marzieh ; Haun, Jered B. ( February 2022 , Frontiers in Bioengineering and Biotechnology)

   The dissociation of tissue and cell aggregates into single cells is of high interest for single cell analysis studies, primary cultures, tissue engineering, and regenerative medicine. However, current methods are slow, poorly controlled, variable, and can introduce artifacts. We previously developed a microfluidic device that contains two separate dissociation modules, a branching channel array and nylon mesh filters, which was used as a polishing step after tissue processing with a microfluidic digestion device. Here, we employed the integrated disaggregation and filtration (IDF) device as a standalone method with both cell aggregates and traditionally digested tissue to perform a well-controlled and detailed study into the effect of mechanical forces on dissociation, including modulation of flow rate, device pass number, and even the mechanism. Using a strongly cohesive cell aggregate model, we found that single cell recovery was highest using flow rates exceeding 40 ml/min and multiple passes through the filter module, either with or without the channel module. For minced and digested kidney tissue, recovery of diverse cell types was maximal using multiple passes through the channel module and only a single pass through the filter module. Notably, we found that epithelial cell recovery from the optimized IDF device alone exceeded our previous efforts, and this result was maintained after reducing digestion time to 20 min. However, endothelial cells and leukocytes still required extended digestion time for maximal recover. These findings highlight the significance of parameter optimization to achieve the highest cell yield and viability based on tissue sample size, extracellular matrix content, and strength of cell-cell interactions. 

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