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Title: The RanBP2 zinc finger domains of chloroplast RNA editing factor OZ1 are required for protein–protein interactions and conversion of C to U
Award ID(s):
1615393
NSF-PAR ID:
10389365
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
The Plant Journal
Volume:
109
Issue:
1
ISSN:
0960-7412
Page Range / eLocation ID:
215 to 226
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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  1. Abstract

    Fatty acid biosynthesis (FAB) is an essential and highly conserved metabolic pathway. In bacteria, this process is mediated by an elaborate network of protein•protein interactions (PPIs) involving a small, dynamic acyl carrier protein that interacts with dozens of other partner proteins (PPs). These PPIs have remained poorly characterized due to their dynamic and transient nature. Using a combination of solution-phase NMR spectroscopy and protein-protein docking simulations, we report a comprehensive residue-by-residue comparison of the PPIs formed during FAB inEscherichia coli. This technique describes and compares the molecular basis of six discrete binding events responsible forE. coliFAB and offers insights into a method to characterize these events and those in related carrier protein-dependent pathways.

     
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  2. null (Ed.)