Microbial sulfur cycling in marine sediments often occurs in environments characterized by transient chemical gradients that affect both the availability of nutrients and the activity of microbes. High turnover rates of intermediate valence sulfur compounds and the intermittent availability of oxygen in these systems greatly impact the activity of sulfur‐oxidizing micro‐organisms in particular. In this study, the thiosulfate‐oxidizing hydrothermal vent bacterium
Marine oxygen‐deficient zones represent a natural source of nitrous oxide (N2O), a potent greenhouse gas and ozone‐depleting agent. To investigate controls on N2O production, the responses of ammonia oxidation (AO) to nitrite () and N2O with respect to oxygen (O2), ammonium () and concentrations were evaluated using tracer incubations in the Eastern Tropical North Pacific. Within the oxycline, additions of and O2stimulated N2O production according to Michaelis–Menten kinetics, indicating that both substrates were limiting, and that N2O production, even if the exact mechanisms remain uncertain, is mediated by predictable kinetics. Low half‐saturation constants for (12–28 nM) and O2(460 ± 130 nM) during N2O production indicate that AO communities are well adapted to low concentrations of both substrates. Hybrid N2O formation (i.e., from one and one unlabeled nitrogen (N) source, e.g., , NO) accounted for ~ 90% of the N2O production from and was robust across the different O2, , and conditions. Lack of response to variable substrate concentrations implies that the unlabeled N source was not limiting for N2O production. Although both O2and were key modulators of N2O production rates, N2O yield (N2O produced per produced) seemed to be controlled solely by O2. The N2O yield increased when O2concentrations dropped below the half‐saturation concentration for AO to (< 1.4
- NSF-PAR ID:
- 10397069
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Limnology and Oceanography
- Volume:
- 68
- Issue:
- 2
- ISSN:
- 0024-3590
- Format(s):
- Medium: X Size: p. 424-438
- Size(s):
- ["p. 424-438"]
- Sponsoring Org:
- National Science Foundation
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