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1. Rainfall pulses increased short-term biocrust chlorophyll but not fungal abundance or N availability in a long-term dryland rainfall manipulation experiment

   https://doi.org/10.1016/j.soilbio.2019.107693  

   Kwiecinski, Jarek V. ; Stricker, Eva ; Sinsabaugh, Robert L. ; Collins, Scott L. ( March 2020 , Soil Biology and Biochemistry)
2. Universal Constraints on Protein Evolution in the Long-Term Evolution Experiment with Escherichia coli

   https://doi.org/10.1093/gbe/evab070  

   Maddamsetti, Rohan ( June 2021 , Genome Biology and Evolution)

   Golding, Brian (Ed.)

   Abstract Although it is well known that abundant proteins evolve slowly across the tree of life, there is little consensus for why this is true. Here, I report that abundant proteins evolve slowly in the hypermutator populations of Lenski’s long-term evolution experiment with Escherichia coli (LTEE). Specifically, the density of all observed mutations per gene, as measured in metagenomic time series covering 60,000 generations of the LTEE, significantly anticorrelates with mRNA abundance, protein abundance, and degree of protein–protein interaction. The same pattern holds for nonsynonymous mutation density. However, synonymous mutation density, measured across the LTEE hypermutator populations, positively correlates with protein abundance. These results show that universal constraints on protein evolution are visible in data spanning three decades of experimental evolution. Therefore, it should be possible to design experiments to answer why abundant proteins evolve slowly.
3. Decreased Soil Organic Matter in a Long‐Term Soil Warming Experiment Lowers Soil Water Holding Capacity and Affects Soil Thermal and Hydrological Buffering

   https://doi.org/10.1029/2019JG005158  

   Werner, W. J. ; Sanderman, J. ; Melillo, J. M. ( April 2020 , Journal of Geophysical Research: Biogeosciences)
4. Weed Diversity Affects Soybean and Maize Yield in a Long Term Experiment in Michigan, USA

   https://doi.org/10.3389/fpls.2017.00236  

   Ferrero, Rosana ; Lima, Mauricio ; Davis, Adam S. ; Gonzalez-Andujar, Jose L. ( February 2017 , Frontiers in Plant Science)
5. Divergent Evolution of Mutation Rates and Biases in the Long-Term Evolution Experiment with Escherichia coli

   https://doi.org/10.1093/gbe/evaa178  

   Maddamsetti, Rohan ; Grant, Nkrumah A ( September 2020 , Genome Biology and Evolution)

   Zhang, George (Ed.)

   Abstract All organisms encode enzymes that replicate, maintain, pack, recombine, and repair their genetic material. For this reason, mutation rates and biases also evolve by mutation, variation, and natural selection. By examining metagenomic time series of the Lenski long-term evolution experiment (LTEE) with Escherichia coli (Good BH, McDonald MJ, Barrick JE, Lenski RE, Desai MM. 2017. The dynamics of molecular evolution over 60,000 generations. Nature 551(7678):45–50.), we find that local mutation rate variation has evolved during the LTEE. Each LTEE population has evolved idiosyncratic differences in their rates of point mutations, indels, and mobile element insertions, due to the fixation of various hypermutator and antimutator alleles. One LTEE population, called Ara+3, shows a strong, symmetric wave pattern in its density of point mutations, radiating from the origin of replication. This pattern is largely missing from the other LTEE populations, most of which evolved missense, indel, or structural mutations in topA, fis, and dusB—loci that all affect DNA topology. The distribution of mutations in those genes over time suggests epistasis and historical contingency in the evolution of DNA topology, which may have in turn affected local mutation rates. Overall, the replicate populations of the LTEE have largely diverged in their mutationmore »rates and biases, even though they have adapted to identical abiotic conditions.« less