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Insect wings are living, flexible structures composed of tubular veins and thin wing membrane. Wing veins can contain hemolymph (insect blood), tracheae, and nerves. Continuous flow of hemolymph within insect wings ensures that sensory hairs, structural elements such as resilin, and other living tissue within the wings remains functional. While it is well known that hemolymph circulates through insect wings, the extent of wing circulation (e.g., whether flow is present in every vein, and whether it is confined to the veins alone) is not well understood, especially for wings with complex wing venation. Over the last 100 years, scientists have developed experimental methods including microscopy, fluorescence, and thermography to observe flow in the wings. Recognizing and evaluating the importance of hemolymph movement in insect wings is critical in evaluating how the wings function both as flight appendages, as active sensors, and as thermoregulatory organs. In this review, we discuss the history of circulation in wings, past and present experimental techniques for measuring hemolymph, and broad implications for the field of hemodynamics in insect wings.
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Complex hemolymph circulation patterns in grasshopper wings
Abstract An insect’s living systems—circulation, respiration, and a branching nervous system—extend from the body into the wing. Wing hemolymph circulation is critical for hydrating tissues and supplying nutrients to living systems such as sensory organs across the wing. Despite the critical role of hemolymph circulation in maintaining healthy wing function, wings are often considered “lifeless” cuticle, and flows remain largely unquantified. High-speed fluorescent microscopy and particle tracking of hemolymph in the wings and body of the grasshopperSchistocerca americanarevealed dynamic flow in every vein of the fore- and hindwings. The global system forms a circuit, but local flow behavior is complex, exhibiting three distinct types: pulsatile, aperiodic, and “leaky” flow. Thoracic wing hearts pull hemolymph from the wing at slower frequencies than the dorsal vessel; however, the velocity of returning hemolymph (in the hindwing) is faster than in that of the dorsal vessel. To characterize the wing’s internal flow mechanics, we mapped dimensionless flow parameters across the wings, revealing viscous flow regimes. Wings sustain ecologically important insect behaviors such as pollination and migration. Analysis of the wing circulatory system provides a template for future studies investigating the critical hemodynamics necessary to sustaining wing health and insect flight.
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- Award ID(s):
- 1812215
- PAR ID:
- 10402826
- Publisher / Repository:
- Nature Publishing Group
- Date Published:
- Journal Name:
- Communications Biology
- Volume:
- 6
- Issue:
- 1
- ISSN:
- 2399-3642
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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