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Title: Atomistic Insights Into The Mechanism of Dual Affinity Switching In Plant Nitrate Transporter NRT1.1
Improving nitrogen use efficiency is critical to enhancing agricultural productivity and to mitigate environmental pollution. To overcome the fluctuations in soil nitrate concentration, plants have evolved an elaborate nitrate transporting mechanism that switches between high and low affinity. In plants, NRT1.1, a root-associated nitrate transporter, switches its affinity upon phosphorylation at Thr101. However, the molecular basis of this unique functional behavior known as dual-affinity switching remains elusive. Crystal structures of the NRT1.1 nitrate transporter have provided evidence for the two competing hypotheses to explain the origin of dual-affinity switching. It is not known how the interplay between transporter phosphorylation and dimerization regulates the affinity switching. To reconcile the different hypotheses, we have performed extensive simulations of nitrate transporter in conjunction with Markov state models to elucidate the molecular origin for a dual-affinity switching mechanism. Simulations of monomeric transporter reveal that phosphorylation stabilizes the outward-facing state and accelerates dynamical transitions for facilitating transport. On the other hand, phosphorylation of the transporter dimer decouples dynamic motions of dimer into independent monomers and thus facilitates substrate transport. Therefore, the phosphorylation-induced enhancement of substrate transport and dimer decoupling not only reconcile the competing experimental results but also provide an atomistic view of how nitrate transport is regulated in plants.  more » « less
Award ID(s):
1845606
PAR ID:
10407603
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
bioRxiv
ISSN:
2692-8205
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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