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1. Micro-contextual identification of archaeological lipid biomarkers using resin-impregnated sediment slabs

   https://doi.org/10.1038/s41598-020-77257-x  

   Rodríguez de Vera, Caterina; Herrera-Herrera, Antonio V.; Jambrina-Enríquez, Margarita; Sossa-Ríos, Santiago; González-Urquijo, Jesús; Lazuen, Talia; Vanlandeghem, Marine; Alix, Claire; Monnier, Gilliane; Pajović, Goran; et al (November 2020, Scientific Reports)

   Abstract Characterizing organic matter preserved in archaeological sediment is crucial to behavioral and paleoenvironmental investigations. This task becomes particularly challenging when considering microstratigraphic complexity. Most of the current analytical methods rely on loose sediment samples lacking spatial and temporal resolution at a microstratigraphic scale, adding uncertainty to the results. Here, we explore the potential of targeted molecular and isotopic biomarker analysis on polyester resin-impregnated sediment slabs from archaeological micromorphology, a technique that provides microstratigraphic control. We performed gas chromatography–mass spectrometry (GC–MS) and gas chromatography–isotope ratio mass spectromety (GC–IRMS) analyses on a set of samples including drill dust from resin-impregnated experimental and archaeological samples, loose samples from the same locations and resin control samples to assess the degree of interference of polyester resin in the GC–MS and Carbon-IRMS signals of different lipid fractions (n-alkanes, aromatics, n-ketones, alcohols, fatty acids and other high polarity lipids). The results show that biomarkers within the n-alkane, aromatic, n-ketone, and alcohol fractions can be identified. Further work is needed to expand the range of identifiable lipid biomarkers. This study represents the first micro-contextual approach to archaeological lipid biomarkers and contributes to the advance of archaeological science by adding a new method to obtain behavioral or paleoenvironmental proxies. 

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2. Proteomic Insights into Psychrophile Growth in Perchlorate-Amended Subzero Conditions: Implications for Martian Life Detection

   https://doi.org/10.1089/ast.2024.0065  

   Gentilhomme, Anais S; Dhakar, Kusum; Timmins-Schiffman, Emma; Chaw, Matthew; Firth, Erin; Junge, Karen; Nunn, Brook L (March 2025, Astrobiology)

   Since the discovery of perchlorates in martian soils, astrobiologists have been curious if and how life could survive in these low-water, high-salt environments. Perchlorates induce chaotropic and oxidative stress but can also confer increased cold tolerance in some extremophiles. Though bacterial survival has been demonstrated at subzero temperatures and in perchlorate solution, proteomic analysis of cells growing in an environment like martian regolith brines-perchlorate with subzero temperatures-has yet to be demonstrated. By defining biosignatures of survival and growth in perchlorate-amended media at subzero conditions, we move closer to understanding the mechanisms that underlie the feasibility of life on Mars. Colwellia psychrerythraea str. 34H (Cp34H), a marine psychrophile, was exposed to perchlorate ions in the form of a diluted Phoenix Mars Lander Wet Chemistry Laboratory solution at -1°C and -5°C. At both temperatures in perchlorate-amended media, Cp34H grew at reduced rates. Mass spectrometry-based proteomics analyses revealed that proteins responsible for mitigating effects of oxidative and chaotropic stress increased, while cellular transport proteins decreased. Cumulative protein signatures suggested modifications to cell-cell or cell-surface adhesion properties. These physical and biochemical traits could serve as putative identifiable biosignatures for life detection in martian environments. 

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3. Lipid biochemical diversity and dynamics reveal phytoplankton nutrient-stress responses and carbon export mechanisms in mesoscale eddies in the North Pacific Subtropical Gyre

   https://doi.org/10.3389/fmars.2024.1427524  

   Bent, Shavonna M; Muratore, Daniel; Becker, Kevin W; Barone, Benedetto; Clemente, Tara; Fredricks, Helen F; Holm, Henry C; Karl, David M; Van_Mooy, Benjamin_A S (September 2024, Frontiers in Marine Science)

   Mesoscale eddies cause deviations from the background physical and biogeochemical states of the oligotrophic oceans, but how these perturbations manifest in microbial ecosystem functioning, such as community macromolecular composition or carbon export, remains poorly characterized. We present comparative lipidomes from communities entrained in two eddies of opposite polarities (cyclone–anticyclone) in the North Pacific Subtropical Gyre (NPSG). A previous work on this two-eddy system has shown differences in particulate inorganic carbon (PIC) and biogenic silica sinking fluxes between the two eddies despite comparable total organic carbon fluxes. We measured the striking differences between the lipidomes of suspended and sinking particles that indicate taxon-specific responses to mesoscale perturbations. Specifically, cyanobacteria did not appear to respond to increased concentrations of phosphorus in the subsurface of the cyclonic eddy, while eukaryotic microbes exhibit P-stress relief as reflected in their lipid signatures. Furthermore, we found that two classes of lipids drive differences between suspended and sinking material: sinking particles are comparatively enriched in phosphatidylcholine (PC, a membrane-associated lipid) and triacylglycerol (TAG, an energy storage lipid). We observed significantly greater export of TAGs from the cyclonic eddy as compared to the anticyclone and found that this flux is strongly correlated with the concentration of ballast minerals (PIC and biogenic silica). This increased export of TAGs from the cyclone, but not the anticyclone, suggests that cyclonic eddy perturbations may be a mechanism for the delivery of energy-rich organic material below the euphotic zone. 

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4. Liposome Formulation for Tumor-Targeted Drug Delivery Using Radiation Therapy

   https://doi.org/10.3390/ijms231911662  

   Stolarz, Amanda J.; Chhetri, Bijay P.; Borrelli, Michael J.; Jenkins, Samir V.; Jamshidi-Parsian, Azemat; Phillips, Joshua H.; Fologea, Daniel; Gandy, Jay; Griffin, Robert J. (October 2022, International Journal of Molecular Sciences)

   Targeted delivery of drugs or other therapeutic agents through internal or external triggers has been used to control and accelerate the release from liposomal carriers in a number of studies, but relatively few utilize energy of therapeutic X-rays as a trigger. We have synthesized liposomes that are triggered by ionizing radiation (RTLs) to release their therapeutic payload. These liposomes are composed of natural egg phosphatidylethanolamine (PE), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, and 1,2-disteroyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG-2000), and the mean size of the RTL was in the range of 114 to 133 nm, as measured by nanoparticle tracking analysis (NTA). The trigger mechanism is the organic halogen, chloral hydrate, which is known to generate free protons upon exposure to ionizing radiation. Once protons are liberated, a drop in internal pH of the liposome promotes destabilization of the lipid bilayer and escape of the liposomal contents. In proof of principle studies, we assessed RTL radiation-release of fluorescent tracers upon exposure to a low pH extracellular environment or exposure to X-ray irradiation. Biodistribution imaging before and after irradiation demonstrated a preferential uptake and release of the liposomes and their cargo at the site of local tumor irradiation. Finally, a potent metabolite of the commonly used chemotherapy irinotecan, SN-38, was loaded into RTL along with near infrared (NIR) fluorescent dyes for imaging studies and measuring tumor cell cytotoxicity alone or combined with radiation exposure, in vitro and in vivo. Fully loaded RTLs were found to increase tumor cell killing with radiation in vitro and enhance tumor growth delay in vivo after three IV injections combined with three, 5 Gy local tumor radiation exposures compared to either treatment modality alone. 

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5. Sediment geochemical data from the Bering Shelf and Yukon Delta, Eastern Bering Sea, June 2023

   https://doi.org/10.18739/A2PG1HQ63  

   Repasch, Marisa (June 2023, NSF Arctic Data Center)

   This dataset contains geochemical and grain size measurements of seabed and suspended sediment samples collected from the Bering Shelf and Yukon prodelta in June 2023. These samples were collected during the 6-12 June 2023 Arctic Chief Scientist Training Cruise, which was a short cruise on the R/V Sikuliaq during its transit from Seward, Alaska to Nome, Alaska. The cruise was sponsored by the University National Oceanographic Laboratory System (UNOLS) Arctic Icebreaker Coordinating Committee and the National Science Foundation. These data were generated as a pilot data set to understand the fate of terrestrial organic carbon in the Yukon River delta and adjacent Bering Sea. Seabed sediment was collected at three locations (MC04, MC06, and MC08) using a multicorer. One seabed sediment sample was collected using a van veen grab sampler. Suspended sediment samples were collected using a hand-deployed niskin bottle and transferred into a clean 10-liter cubitainer for transport and temporary storage. Suspended sediment was filtered on the ship within 24 hours of collection using a Geotech barrel filter with a 0.45 micron Polyethersulfone (PES) membrane filter. Multicores were extruded on deck and subsamples were transferred to sterile whirl-pak sample bags. All sediment samples were frozen and subsequently shipped back to the lab in coolers. Sediment samples were analyzed for grain size, bulk elemental composition via X-ray fluorescence (XRF), and organic carbon and nitrogen concentrations and isotopes via EA-IRMS (elemental analyzer-isotope ratio mass spectrometry). Grain size distributions were measured using a Malvern mastersizer laser diffraction particle size analyzer at the University of Colorado Boulder. Bulk elemental composition was measured on the Rigaku XRF in the Analytical Geochemistry Lab at the University of New Mexico. Total organic carbon (TOC), total nitrogen (TN), and carbon/nitrogen isotopes were measured at the Center for Stable Isotopes at the University of New Mexico. Prior to EA-IRMS analyses, sediment samples were acid-treated to remove inorganic carbon following the method of Galy et al., 2007. References Galy, V., Bouchez, J., & France‐Lanord, C. (2007). Determination of total organic carbon content and δ13C in carbonate‐rich detrital sediments. Geostandards and Geoanalytical research, 31(3), 199-207. 

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