The present work reports the synthesis of water-dispersible polypyrrole (WD-PPy) and polythiophene (WD-PTh) copolymers in different weight ratios and their characterization using experimental and theoretical techniques. The copolymers were spectroscopically characterized using experimental 13 C-NMR, FTIR, and UV-visible studies, and theoretical FTIR and UV-visible studies. The theoretical frequency and UV-visible data were computed using Gaussian 09 software with the functional DFT/B3LYP method and 6-31G(d) basis set. For the first time, biophysical interaction studies were carried out using bovine serum albumin (BSA) and human serum albumin (HSA) for these polymers which are not yet reported in the literature. The results showed strong binding of the co-oligomers with BSA/HSA which could be utilized in designing potent inhibitors and biosensors.
This content will become publicly available on January 1, 2024
Experimental and biophysical interaction studies of alanine modified polyaniline with bovine serum albumin and human serum albumin: Influence of alanine modification on the spectral, morphological and electronic properties
- Award ID(s):
- 2122044
- Publication Date:
- NSF-PAR ID:
- 10412191
- Journal Name:
- Synthetic Metals
- Volume:
- 292
- Issue:
- C
- Page Range or eLocation-ID:
- 117248
- ISSN:
- 0379-6779
- Sponsoring Org:
- National Science Foundation
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We report separation of genomic DNA (48 kbp) from bovine serum albumin (BSA) by the electro-hydrodynamic coupling between a pressure-driven flow and a parallel electric field. Electro-hydrodynamic extraction exploits this coupling to trap DNA molecules at the entrance of a microfluidic contraction channel, while allowing proteins and salts to be flushed from the device. Samples (10 μL) containing λ-DNA (1 ng) and BSA (0.3 mg) were injected directly into the device and convected to the contraction channel entrance by a flowing buffer solution. The DNA remains trapped in this region essentially indefinitely, while proteins and salts are eluted. The effectiveness of the concept has been assessed by fluorescence measurements of DNA and BSA concentrations. Electro-hydrodynamic extraction in a single-stage device was found to enhance the concentration of DNA 40-fold, while reducing the BSA concentration by four orders of magnitude. The relative concentrations of DNA to BSA at the contraction channel entrance can be as large as 1.5 : 1, corresponding to an A260/280 ratio of 1.9. The maximum yield of DNA from a salt-free solution is 50%, while salted (150 mM) solutions have a lower yield (38%).
