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Title: TERMIS AM
Human induced pluripotent stem cell (hiPSC)-derived brain spheroids can recapitulate the complex cytoarchitecture of the brain as well as the genetic/epigenetic footprint of human brain development. Although the brain spheroids can mimic the structures and functions of the brain in vivo at certain complexity, the 3D models do not have a perfusable microvascular network that can provide the interaction with spheroids. Here we report on a microfluidic-based three-dimensional, cortical spheroid tissue grafted on the vascular-network. Angiogenic sprouting was induced by using concentration gradient-driven angiogenic factors and its vascularized network was characterized in terms of morphology, directional alignment under perfusion, lumen formation, and permeability. This paper demonstrates the potential utility of a membrane-free in vitro cortical spheroid tissue construct with perfusable microvascular network that can be scaled up to a high throughput platform as a cost-effective alternative platform to model brain diseases and disorders.  more » « less
Award ID(s):
2100987
PAR ID:
10430431
Author(s) / Creator(s):
Date Published:
Journal Name:
Development of Cortical Spheroid Tissue Constructs with Perfusable Microvasculature Platform
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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