An official website of the United States government
Imaging beyond the diffraction limit barrier has attracted wide attention due to the ability to resolve previously hidden image features. Of the various super-resolution microscopy techniques available, a particularly simple method called saturated excitation microscopy (SAX) requires only simple modification of a laser scanning microscope: The illumination beam power is sinusoidally modulated and driven into saturation. SAX images are extracted from the harmonics of the modulation frequency and exhibit improved spatial resolution. Unfortunately, this elegant strategy is hindered by the incursion of shot noise that prevents high-resolution imaging in many realistic scenarios. Here, we demonstrate a technique for super-resolution imaging that we call computational saturated absorption (CSA) in which a joint deconvolution is applied to a set of images with diversity in spatial frequency support among the point spread functions (PSFs) used in the image formation with saturated laser scanning fluorescence microscopy. CSA microscopy allows access to the high spatial frequency diversity in a set of saturated effective PSFs, while avoiding image degradation from shot noise.
more »
« less
Single-shot spatial frequency modulation for imaging
Spatial frequency modulation for imaging (SPIFI) has traditionally employed a time-varying spatial modulation of the excitation beam. Here, for the first time to our knowledge, we introduce single-shot SPIFI, where the spatial frequency modulation is imposed across the entire spatial bandwidth of the optical system simultaneously enabling single-shot operation.
more »
« less
- PAR ID:
- 10430584
- Publisher / Repository:
- Optical Society of America
- Date Published:
- Journal Name:
- Optics Express
- Volume:
- 31
- Issue:
- 15
- ISSN:
- 1094-4087; OPEXFF
- Format(s):
- Medium: X Size: Article No. 24283
- Size(s):
- Article No. 24283
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
n/a (Ed.)We experimentally explore single-shot state identification using long alphabets of states and employing different modulation schemes. We use time-resolved quantum measurement and Bayesian inference to identify the input state and demonstrate the advantage of this single-shot measurement over classical state identification. For each single-shot measurement, we estimate the confidence of state identification based on the quantum measurement and demonstrate the physical significance of confidence estimates. Particularly, we show that a set of confidence values correctly represents the probabilities of successful state identification for a given experimental outcome. We investigate the alphabets of coherent states with different modulations and show that confidence estimates yield the reliability of each act of measurement independently of the modulation used.more » « less
-
Ultrafast laser pulse beams are four-dimensional, space–time phenomena that can exhibit complicated, coupled spatial and temporal profiles. Tailoring the spatiotemporal profile of an ultrafast pulse beam is necessary to optimize the focused intensity and to engineer exotic spatiotemporally shaped pulse beams. Here we demonstrate a single-pulse, reference-free spatiotemporal characterization technique based on two colocated synchronized measurements: (1) broadband single-shot ptychography and (2) single-shot frequency resolved optical gating. We apply the technique to measure the nonlinear propagation of an ultrafast pulse beam through a fused silica window. Our spatiotemporal characterization method represents a major contribution to the growing field of spatiotemporally engineered ultrafast laser pulse beams.more » « less
-
Abstract Detection of long wave infrared (LWIR) light at room temperature is a long‐standing challenge due to the low energy of photons. A low‐cost, high‐performance LWIR detector or camera that operates under such conditions is pursued for decades. Currently, all available detectors operate based on amplitude modulation (AM) and are limited in performance by AM noises, including Johnson noise, shot noise, and background fluctuation noise. To address this challenge, a frequency modulation (FM)‐based detection technique is introduced, which offers inherent robustness against different types of AM noises. The FM‐based approach yields an outstanding room temperature noise equivalent power (NEP), response time, and detectivity (D*). This result promises a novel uncooled LWIR detection scheme that is highly sensitive, low‐cost, and can be easily integrated with electronic readout circuitry, without the need for complex hybridization.more » « less
-
Abstract Attosecond science has demonstrated that electrons can be controlled on the sub-cycle time scale of an optical waveform, paving the way towards optical frequency electronics. However, these experiments historically relied on high-energy laser pulses and detection not suitable for microelectronic integration. For practical optical frequency electronics, a system suitable for integration and capable of generating detectable signals with low pulse energies is needed. While current from plasmonic nanoantenna emitters can be driven at optical frequencies, low charge yields have been a significant limitation. In this work we demonstrate that large-scale electrically connected plasmonic nanoantenna networks, when driven in concert, enable charge yields sufficient for single-shot carrier-envelope phase detection at repetition rates exceeding tens of kilohertz. We not only show that limitations in single-shot CEP detection techniques can be overcome, but also demonstrate a flexible approach to optical frequency electronics in general, enabling future applications such as high sensitivity petahertz-bandwidth electric field sampling or logic-circuits.more » « less
