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These data represent the abundance, biovolume, and biomass of prokaryotic phytoplankton, eukaryotic pico- and nano- phytoplankton, and heterotrophic bacteria from discrete flow cytometry samples collected during the Northeast U.S. Shelf Long-Term Ecological Research (NES-LTER) Transect cruises, ongoing since 2018. Samples were collected and preserved from the water column at multiple depths using Niskin bottles on a CTD rosette system along the NES-LTER transect, and analyzed post cruise. Cells were identified and enumerated from the flow cytometry data files based on their scattering, SYBR (525 nm), phycoerythrin (575 nm) and chlorophyll (680 nm) fluorescence signals. Gating was completed manually in the Attune NXT software interface.
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Abundance, biovolume, and biomass of Synechococcus and eukaryote pico- and nano- plankton from continuous underway flow cytometry during NES-LTER Transect cruises, ongoing since 2018
These data represent the abundance, biovolume, and biomass of prokaryotic and eukaryotic picoplankton and nanoplankton sampled continuously underway during Northeast U.S. Shelf Long-Term Ecological Research (NES-LTER) Transect cruises, ongoing since 2018. Samples were obtained with an Attune NxT Flow Cytometer sampling at approximately 2-min intervals from the underway science seawater. Cells were identified and enumerated from the flow cytometry data files based on their scattering, phycoerythrin (575 nm) and chlorophyll (680 nm) fluorescence signals.
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- PAR ID:
- 10439678
- Publisher / Repository:
- Environmental Data Initiative
- Date Published:
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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The continuous underway fluorescence, induced by in vivo chlorophyll-a (Chl-a), of surface waters of the Northeast U.S. shelf is compared to discrete Chl-a samples for post-calibration, collected ship-board as part of the Northeast U.S. Shelf Long-Term Ecological Research (NES LTER). Chl-a values derived from the manufacturer-calibrated sensors (hereafter, “continuous fluorescence”) and collected continuously are often different from the precise Chl-a concentrations obtained from discrete, extracted samples. Moreover, underway fluorometers and manufacturer calibrations differ per cruise. Thus, post-calibration of the continuous fluorescence signals using discrete Chl-a measurements is essential to standardize and compare the high-resolution underway Chl-a data along cruise tracks. For six cruises aboard the R/V Endeavor between summer 2019 and summer 2021, 12 to 22 discrete samples were collected from the underway system to measure Chl-a concentrations. These discrete Chl-a concentrations were then compared, using simple linear regressions (Model I least square fit), to corresponding continuous fluorescence values recorded by the two independent fluorometers installed with the underway system. For each cruise a preferred fluorometer was identified based on the best fit of the linear regression between discrete Chl-a concentrations and continuous fluorescence values. The slope and the intercept of the linear regression were used to post-calibrate continuous fluorescence values into standardized and intercomparable Chl-a concentration. This data package includes a table for the underway discrete Chl-a values and a table for the 1-min post-calibrated continuous fluorescence values for the preferred underway fluorometer per cruise.more » « less
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