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Title: Structure and Mechanics of Dynein Motors
Dyneins make up a family of AAA+ motors that move toward the minus end of microtubules. Cytoplasmic dynein is responsible for transporting intracellular cargos in interphase cells and mediating spindle assembly and chromosome positioning during cell division. Other dynein isoforms transport cargos in cilia and power ciliary beating. Dyneins were the least studied of the cytoskeletal motors due to challenges in the reconstitution of active dynein complexes in vitro and the scarcity of high-resolution methods for in-depth structural and biophysical characterization of these motors. These challenges have been recently addressed, and there have been major advances in our understanding of the activation, mechanism, and regulation of dyneins. This review synthesizes the results of structural and biophysical studies for each class of dynein motors. We highlight several outstanding questions about the regulation of bidirectional transport along microtubules and the mechanisms that sustain self-coordinated oscillations within motile cilia.  more » « less
Award ID(s):
1954449
PAR ID:
10443759
Author(s) / Creator(s):
; ; ; ;
Date Published:
Journal Name:
Annual Review of Biophysics
Volume:
50
Issue:
1
ISSN:
1936-122X
Page Range / eLocation ID:
549 to 574
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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