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Hydrogels are candidate building blocks in a wide range of biomaterial applications including soft and biohybrid robotics, microfluidics, and tissue engineering. Recent advances in embedded 3D printing have broadened the design space accessible with hydrogel additive manufacturing. Specifically, the Freeform Reversible Embedding of Suspended Hydrogels (FRESH) technique has enabled the fabrication of complex 3D structures using extremely soft hydrogels, e.g., alginate and collagen, by assembling hydrogels within a fugitive support bath. However, the low structural rigidity of FRESH printed hydrogels limits their applications, especially those that require operation in nonaqueous environments. In this study, we demonstrated long-fiber embedded hydrogel 3D printing using a multihead printing platform consisting of a custom-built fiber extruder and an open-source FRESH bioprinter with high embedding fidelity. Using this process, fibers were embedded in 3D printed hydrogel components to achieve significant structural reinforcement (e.g., tensile modulus improved from 56.78 ± 8.76 to 382.55 ± 25.29 kPa and tensile strength improved from 9.44 ± 2.28 to 45.05 ± 5.53 kPa). In addition, we demonstrated the versatility of this technique by using fibers of a wide range of sizes and material types and implementing different 2D and 3D embedding patterns, such as embedding a conical helix using electrochemically aligned collagen fiber via nonplanar printing. Moreover, the technique was implemented using low-cost material and is compatible with open-source software and hardware, which facilitates its adoption and modification for new research applications.
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A Power Compensation Strategy for Achieving Homogeneous Microstructures for 4D Printing Shape-Adaptive PNIPAM Hydrogels: Out-of-Plane Variations
In the last decade, 3D printing has attracted significant attention and has resulted in benefits to many research areas. Advances in 3D printing with smart materials at the microscale, such as hydrogels and liquid crystalline polymers, have enabled 4D printing and various applications in microrobots, micro-actuators, and tissue engineering. However, the material absorption of the laser power and the aberrations of the laser light spot will introduce a decay in the polymerization degree along the height direction, and the solution to this problem has not been reported yet. In this paper, a compensation strategy for the laser power is proposed to achieve homogeneous and high aspect ratio hydrogel structures at the microscale along the out-of-plane direction. Linear approximations for the power decay curve are adopted for height steps, discretizing the final high aspect ratio structures. The strategy is achieved experimentally with hydrogel structures fabricated by two-photon polymerization. Moreover, characterizations have been conducted to verify the homogeneity of the printed microstructures. Finally, the saturation of material property is investigated by an indirect 3D deformation method. The proposed strategy is proved to be effective and can be explored for other hydrogel materials showing significant deformation. Furthermore, the strategy for out-of-plane variations provides a critical technique to achieve 4D-printed homogeneous shape-adaptive hydrogels for further applications.
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- PAR ID:
- 10444269
- Date Published:
- Journal Name:
- Gels
- Volume:
- 8
- Issue:
- 12
- ISSN:
- 2310-2861
- Page Range / eLocation ID:
- 828
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.3390/gels8120828
