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Title: Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles
Hydrogels are attractive materials for tissue engineering, but efforts to date have shown limited ability to produce the microstructural features necessary to promote cellular self-organization into hierarchical three-dimensional (3D) organ models. Here we develop a hydrogel ink containing prefabricated gelatin fibres to print 3D organ-level scaffolds that recapitulate the intra- and intercellular organization of the heart. The addition of prefabricated gelatin fibres to hydrogels enables the tailoring of the ink rheology, allowing for a controlled sol–gel transition to achieve precise printing of free-standing 3D structures without additional supporting materials. Shear-induced alignment of fibres during ink extrusion provides microscale geometric cues that promote the self-organization of cultured human cardiomyocytes into anisotropic muscular tissues in vitro. The resulting 3D-printed ventricle in vitro model exhibited biomimetic anisotropic electrophysiological and contractile properties.  more » « less
Award ID(s):
2011754
NSF-PAR ID:
10501536
Author(s) / Creator(s):
; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publisher / Repository:
Nature
Date Published:
Journal Name:
Nature Materials
Volume:
22
Issue:
8
ISSN:
1476-1122
Page Range / eLocation ID:
1039 to 1046
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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