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Title: Enhanced resolution dispersion optimized multiphoton microscopy with spatial frequency modulation imaging
Using the structured illumination, single pixel detection imaging technique SPatIal Frequency modulation Imaging (SPIFI), we demonstrate a cascaded Wavelength Domain and Spatial Domain (WD-SD-SPIFI) system enabling real-time, in-line, second order dispersion compensation optimization for multiphoton imaging. Enhanced resolution is demonstrated by imaging a sub-diffractive 140 nm fluorescent nanodiamond with Two Photon Excitation Fluorescence (2PEF) to measure the Point Spread Function (PSF). With a 1034 nm pulsed laser through a Numerical Aperture (NA) of 0.5, a PSF Full Width at Half Max (FWHM) of 780 nm was measured with minimal post processing analysis that only requires Fast Fourier Transforms (FFTs).  more » « less
Award ID(s):
1934288 1707287
PAR ID:
10450494
Author(s) / Creator(s):
; ; ; ;
Editor(s):
Periasamy, Ammasi; So, Peter T.; König, Karsten
Date Published:
Journal Name:
PROCEEDINGS OF SPIE
Page Range / eLocation ID:
62
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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