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Abstract Uncertainty about long‐term leaf‐level responses to atmospheric CO2rise is a major knowledge gap that exists because of limited empirical data. Thus, it remains unclear how responses of leaf gas exchange to elevated CO2(eCO2) vary among plant species and functional groups, or across different levels of nutrient supply, and whether they persist over time for long‐lived perennials. Here, we report the effects of eCO2on rates of net photosynthesis and stomatal conductance in 14 perennial grassland species from four functional groups over two decades in a Minnesota Free‐Air CO2Enrichment experiment, BioCON. Monocultures of species belonging to C3grasses, C4grasses, forbs, and legumes were exposed to two levels of CO2and nitrogen supply in factorial combinations over 21 years. eCO2increased photosynthesis by 12.9% on average in C3species, substantially less than model predictions of instantaneous responses based on physiological theory and results of other studies, even those spanning multiple years. Acclimation of photosynthesis to eCO2was observed beginning in the first year and did not strengthen through time. Yet, contrary to expectations, the response of photosynthesis to eCO2was not enhanced by increased nitrogen supply. Differences in responses among herbaceous plant functional groups were modest, with legumes responding the most and C4grasses the least as expected, but did not further diverge over time. Leaf‐level water‐use efficiency increased by 50% under eCO2primarily because of reduced stomatal conductance. Our results imply that enhanced nitrogen supply will not necessarily diminish photosynthetic acclimation to eCO2in nitrogen‐limited systems, and that significant and consistent declines in stomatal conductance and increases in water‐use efficiency under eCO2may allow plants to better withstand drought.
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Interactive effects of elevated CO2 , warming, reduced rainfall, and nitrogen on leaf gas exchange in five perennial grassland species
Abstract Global changes can interact to affect photosynthesis and thus ecosystem carbon capture, yet few multi‐factor field studies exist to examine such interactions. Here, we evaluate leaf gas exchange responses of five perennial grassland species from four functional groups to individual and interactive global changes in an open‐air experiment in Minnesota, USA, including elevated CO2(eCO2), warming, reduced rainfall and increased soil nitrogen supply. All four factors influenced leaf net photosynthesis and/or stomatal conductance, but almost all effects were context‐dependent, i.e. they differed among species, varied with levels of other treatments and/or depended on environmental conditions. Firstly, the response of photosynthesis to eCO2depended on species and nitrogen, became more positive as vapour pressure deficit increased and, for a C4grass and a legume, was more positive under reduced rainfall. Secondly, reduced rainfall increased photosynthesis in three functionally distinct species, potentially via acclimation to low soil moisture. Thirdly, warming had positive, neutral or negative effects on photosynthesis depending on species and rainfall. Overall, our results show that interactions among global changes and environmental conditions may complicate predictions based on simple theoretical expectations of main effects, and that the factors and interactions influencing photosynthesis vary among herbaceous species.
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- PAR ID:
- 10456362
- Publisher / Repository:
- Wiley-Blackwell
- Date Published:
- Journal Name:
- Plant, Cell & Environment
- Volume:
- 43
- Issue:
- 8
- ISSN:
- 0140-7791
- Page Range / eLocation ID:
- p. 1862-1878
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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