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Title: Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants
Summary

Plant oils are valuable commodities for food, feed, renewable industrial feedstocks and biofuels. To increase vegetable oil production, here we show that the nonspecific phospholipase C6 (NPC6) promotes seed oil production in the Brassicaceae seed oil species Arabidopsis, Camelina and oilseed rape.

Overexpression ofNPC6increased seed oil content, seed weight and oil yield both in Arabidopsis and Camelina, whereas knockout ofNPC6decreased seed oil content and seed size. NPC6 is associated with the chloroplasts and microsomal membranes, and hydrolyzes phosphatidylcholine and galactolipids to produce diacylglycerol. Knockout and overexpression of NPC6 decreased and increased, respectively, the flux of fatty acids from phospholipids and galactolipids into triacylglycerol production.

Candidate‐gene association study in oilseed rape indicates that onlyBnNPC6.C01of the four homeologuesNPC6sis associated with seed oil content and yield. Haplotypic analysis indicates that theBnNPC6.C01favorable haplotype can increase both seed oil content and seed yield.

These results indicate that NPC6 promotes membrane glycerolipid turnover to accumulate TAG production in oil seeds and thatNPC6has a great application potential for oil yield improvement.

 
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NSF-PAR ID:
10458790
Author(s) / Creator(s):
 ;  ;  ;  ;  ;  ;  ;  ;  ;  
Publisher / Repository:
Wiley-Blackwell
Date Published:
Journal Name:
New Phytologist
Volume:
226
Issue:
4
ISSN:
0028-646X
Page Range / eLocation ID:
p. 1055-1073
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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