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Title: Establishing microbial co‐cultures for 3‐hydroxybenzoic acid biosynthesis on glycerol
Abstract

Converting renewable feedstocks to aromatic compounds using engineered microbes offers a robust approach for sustainable, environment‐friendly, and cost‐effective production of these value‐added products without the reliance on petroleum. In this study, rationally designedE. coli–E. colico‐culture systems were established for converting glycerol to 3‐hydroxybenzoic acid (3HB). Specifically, the 3HB pathway was modularized and accommodated by two metabolically engineeredE. colistrains. The co‐culture biosynthesis was optimized by using different cultivation temperatures, varying the inoculum ratio between the co‐culture strains, recruitment of a key pathway intermediate transporter, strengthening the critical pathway enzyme expression, and adjusting the timing for inducing pathway gene expression. Compared with theE. colimono‐culture, the optimized co‐culture showed 5.3‐fold improvement for 3HB biosynthesis. This study demonstrated the applicability of modular co‐culture engineering for addressing the challenges of aromatic compound biosynthesis.

 
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Award ID(s):
1706058
NSF-PAR ID:
10461388
Author(s) / Creator(s):
 ;  ;  ;  
Publisher / Repository:
Wiley Blackwell (John Wiley & Sons)
Date Published:
Journal Name:
Engineering in Life Sciences
Volume:
19
Issue:
5
ISSN:
1618-0240
Page Range / eLocation ID:
p. 389-395
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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