Interstitial water or pore water occupies the space between soil particles and provides “hotspots” and “fluvial networks” for microbial activities in floodplain soil. However, to date, we know very little about the microorganisms living in pore water and how they respond to environmental changes. This study aimed to understand microbial distribution and assemblage in riparian pore waters, and how they respond to water chemistry and redox gradients associated with hydrological processes. We analysed the annual changes of porewater microbial communities from the east and west banks of the White Clay Creek, a site at the Christina River Basin – Critical Zone Observatory, Pennsylvania, USA. Microbial abundances were quantified by epifluorescence microscopy and detailed community structures were characterised by high‐throughput sequencing. Water chemistry and redox gradients were also monitored and recorded, and their interactions with porewater microbiomes were analysed using correlations and multivariate analyses. Abundance of microbial cells increased during summer and late autumn. Wetland porewater microbiomes mainly contained Acidobacteria, Bacteroidetes, Nitrospirae and Proteobacteria, and microbiome structures were easily distinguishable from those in the underlying hyporheic gravel layer. Seasonal dynamics of bacterial community structure in the east and west wetlands were distinct, responding to floodplain topography and associated hydrological/geochemical processes. Iron (Fe)‐cycling bacteria (mainly Gallionellaceae and Microbial abundances (cell density) in pore waters showed similar seasonal patterns across stream banks, but microbial community structure did not. Microbiome assembly in pore water is correlated with water chemistry and redox gradients primarily associated with local hydrological processes. As a consequence of their significance for carbon (C) mineralisation and Fe reduction at terrestrial–aquatic interfaces, microbiomes in riparian pore waters and associated microbial activity play an essential role in C and mineral dynamics. These findings will inform future studies of the response of freshwater ecosystems to hydrological dynamics influenced by global climate change.
Changes in streamflow and water table elevation influence oxidation–reduction (redox) conditions near river–aquifer interfaces, with potentially important consequences for solute fluxes and biogeochemical reaction rates. Although continuous measurements of groundwater chemistry can be arduous, in situ sensors reveal chemistry dynamics across a wide range of timescales. We monitored redox potential in an aquifer adjacent to a tidal river and used spectral and wavelet analyses to link redox responses to hydrologic perturbations within the bed and banks. Storms perturb redox potential within both the bed and banks over timescales of days to weeks. Tides drive semidiurnal oscillations in redox potential within the streambed that are absent in the banks. Wavelet analysis shows that tidal redox oscillations in the bed are greatest during late summer (wavelet magnitude of 5.62 mV) when river stage fluctuations are on the order of 70 cm and microbial activity is relatively high. Tidal redox oscillations diminish during the winter (wavelet magnitude of 2.73 mV) when river stage fluctuations are smaller (on the order of 50 cm) and microbial activity is presumably low. Although traditional geochemical observations are often limited to summer baseflow conditions, in situ redox sensing provides continuous, high‐resolution chemical characterization of the subsurface, revealing transport and reaction processes across spatial and temporal scales in aquifers.
more » « less- Award ID(s):
- 1752995
- NSF-PAR ID:
- 10461896
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Hydrological Processes
- Volume:
- 33
- Issue:
- 3
- ISSN:
- 0885-6087
- Page Range / eLocation ID:
- p. 405-413
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Abstract Rhodoferax spp.) dominated the porewater microbiome, and their relative abundance was significantly higher in the east than the west wetland. Furthermore, Fe‐oxidising bacteria (Gallionellaceae) were negatively correlated with Fe‐reducing bacteria (Rhodoferax spp.) at the east wetland. -
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Abstract Tidal freshwater zones (TFZs) are transitional environments between terrestrial and coastal waters. TFZs have freshwater chemistry and tidal physics, and yet are neither river nor estuary based on classic definitions. Such zones have been occasionally discussed in the literature but lack a consistent nomenclature and framework for study. This work proposes a measurable definition for TFZs based on three longitudinal points of interest: (1) the upstream limit of brackish water, (2) the upstream limit of bidirectional tidal velocities, and (3) the upstream limit of tidal stage fluctuations. The resulting size and position of a TFZ is transient and depends on the balance of tidal and riverine forces that evolves over event, tidal, seasonal, and annual (or longer) timescales. The concept, definition, and transient analysis of TFZ position are illustrated using field observations from the Aransas River (Texas, USA) from July 2015 to July 2016. The median Aransas TFZ length was 59.9 km, with a late summer maximum of 66.0 km and a winter minimum of 53.6 km. The TFZ typically (annual median) began 11.8 km upstream from the river mouth (15.4 km winter/11.2 km summer medians) and ended 71.7 km upstream (69.0 km/77.2 km). Seasonally low baseflow in the Aransas River promoted gradual coastal salt encroachment upstream, which shortened the TFZ. However, sporadic large rainfall/runoff events rapidly elongated the TFZ. The TFZ definition establishes a quantifiable framework for analyzing these critical freshwater systems that reside at the nexus of natural and human‐influenced hydrology, tides, and climate.
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null (Ed.)The Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE) is a coordinated, multiexpedition International Ocean Discovery Program (IODP) drilling project designed to investigate fault mechanics and seismogenesis along subduction megathrusts through direct sampling, in situ measurements, and long-term monitoring in conjunction with allied laboratory and numerical modeling studies. The fundamental scientific objectives of the NanTroSEIZE drilling project include characterizing the nature of fault slip and strain accumulation, fault and wall rock composition, fault architecture, and state variables throughout the active plate boundary system. IODP Expedition 365 is part of NanTroSEIZE Stage 3, with the following primary objectives: (1) retrieval of a temporary observatory at Site C0010 that has been monitoring temperature and pore pressure within the major splay thrust fault (termed the “megasplay”) at 400 meters below seafloor since November 2010 and (2) deployment of a complex long-term borehole monitoring system (LTBMS) that will be connected to the Dense Oceanfloor Network System for Earthquakes and Tsunamis (DONET) seafloor cabled observatory network postexpedition (anticipated June 2016). The LTBMS incorporates multilevel pore pressure sensing, a volumetric strainmeter, tiltmeter, geophone, broadband seismometer, accelerometer, and thermistor string. Together with an existing observatory at Integrated Ocean Drilling Program Site C0002 and a possible future installation near the trench, the Site C0010 observatory will allow monitoring within and above regions of contrasting behavior of the megasplay fault and the plate boundary as a whole. These include a site above the updip edge of the locked zone (Site C0002), a shallow site in the megasplay fault zone and its footwall (Site C0010), and a site at the tip of the accretionary prism (Integrated Ocean Drilling Program Site C0006). Together, this suite of observatories has the potential to capture deformation spanning a wide range of timescales (e.g., seismic and microseismic activity, slow slip, and interseismic strain accumulation) across a transect from near-trench to the seismogenic zone. Site C0010 is located 3.5 km along strike to the southwest of Integrated Ocean Drilling Program Site C0004. The site was drilled and cased during Integrated Ocean Drilling Program Expedition 319, with casing screens spanning a ~20 m interval that includes the megasplay fault, and suspended with a temporary instrument package (a “SmartPlug”). During Integrated Ocean Drilling Program Expedition 332 in late 2010, the instrument package was replaced with an upgraded sensor package (the “GeniusPlug”), which included pressure and temperature sensors and a set of geochemical and biological experiments. Expedition 365 achieved its primary scientific and operational objectives, including recovery of the GeniusPlug with a >5 y record of pressure and temperature conditions within the shallow megasplay fault zone, geochemical samples, and its in situ microbial colonization experiment; and installation of the LTBMS. The pressure records from the GeniusPlug include high-quality records of formation and seafloor responses to multiple fault slip events, including the 11 March 2011 Tohoku M9 and 1 April 2016 Mie-ken Nanto-oki M6 earthquakes. The geochemical sampling coils yielded in situ pore fluids from the splay fault zone, and microbes were successfully cultivated from the colonization unit. The complex sensor array, in combination with the multilevel hole completion, is one of the most ambitious and sophisticated observatory installations in scientific ocean drilling (similar to that in Hole C0002G, deployed in 2010). Overall, the installation went smoothly, efficiently, and ahead of schedule. The extra time afforded by the efficient observatory deployment was used for coring in Holes C0010B–C0010E. Despite challenging hole conditions, the depth interval corresponding to the screened casing across the megasplay fault was successfully sampled in Hole C0010C, and the footwall of the megasplay was sampled in Hole C0010E, with >50% recovery for both zones. In the hanging wall of the megasplay fault (Holes C0010C and C0010D), we recovered indurated silty clay with occasional ash layers and sedimentary breccias. Some of the deposits show burrows and zones of diagenetic alteration/colored patches. Mudstones show different degrees of deformation spanning from occasional fractures to intervals of densely fractured scaly claystones of up to >10 cm thickness. Sparse faulting with low displacement (usually <2 cm) is seen in core and exhibits primarily normal and, rarely, reversed sense of slip. When present, ash was entrained along fractures and faults. On one occasion, a ~10 cm thick ash layer was found, which showed a fining-downward gradation into a mottled zone with clasts of the underlying silty claystones. In Hole C0010E, the footwall to the megasplay fault was recovered. Sediments are horizontally to gently dipping and mainly comprise silt of olive-gray color. The deposits of the underthrust sediment prism are less indurated than the hanging wall mudstones and show lamination on a centimeter scale. The material is less intensely deformed than the mudstones, and apart from occasional fracturation (some of it being drilling disturbance), evidence of structural features is absent.more » « less
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null (Ed.)The Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE) is a coordinated, multiexpedition International Ocean Discovery Program (IODP) drilling project designed to investigate fault mechanics and seismogenesis along subduction megathrusts through direct sampling, in situ measurements, and long-term monitoring in conjunction with allied laboratory and numerical modeling studies. The fundamental scientific objectives of the NanTroSEIZE drilling project include characterizing the nature of fault slip and strain accumulation, fault and wall rock composition, fault architecture, and state variables throughout the active plate boundary system. IODP Expedition 365 is part of NanTroSEIZE Stage 3, with the following primary objectives: 1. Retrieval of a temporary observatory at Site C0010 that began monitoring temperature and pore pressure within the major splay thrust fault (termed the “megasplay”) at 400 meters below seafloor in November 2010. 2. Deployment of a complex long-term borehole monitoring system (LTBMS) designed to be connected to the Dense Oceanfloor Network System for Earthquakes and Tsunamis (DONET) seafloor cabled observatory network postexpedition. The LTBMS incorporates multilevel pore pressure sensing, a volumetric strainmeter, tiltmeter, geophone, broadband seismometer, accelerometer, and thermistor string. Together with an existing observatory at Integrated Ocean Drilling Program Site C0002 and a planned future installation near the trench, the Site C0010 observatory allows monitoring within and above regions of contrasting behavior of the megasplay fault and the plate boundary as a whole. These include a site above the updip edge of the locked zone (Site C0002), a shallow site in the megasplay fault zone and its footwall (Site C0010), and a site at the tip of the accretionary prism (possible future installation at Integrated Ocean Drilling Program Site C0006). Together, this suite of observatories has the potential to capture deformation spanning a wide range of timescales (e.g., seismic and microseismic activity, slow slip, and interseismic strain accumulation) across a transect from near-trench to the seismogenic zone. Site C0010 is located 3.5 km along strike to the southwest of Integrated Ocean Drilling Program Site C0004. The site was drilled and cased during Integrated Ocean Drilling Program Expedition 319, with casing screens spanning a ~20 m interval that includes the megasplay fault, and suspended with a temporary instrument package (a “SmartPlug”), which included pressure and temperature sensors. During Integrated Ocean Drilling Program Expedition 332 in late 2010, the instrument package was replaced with an upgraded sensor package (the “GeniusPlug”), which included a set of geochemical and biological experiments in addition to pressure and temperature sensors. Expedition 365 achieved its primary scientific and operational objectives, including recovery of the GeniusPlug with a >5 y record of pressure and temperature conditions within the shallow megasplay fault zone, geochemical samples, and its in situ microbial colonization experiment; and installation of the LTBMS. The pressure records from the GeniusPlug include high-quality records of formation and seafloor responses to multiple fault slip events, including the 11 March 2011 Tohoku M9 and 1 April 2016 Mie-ken Nanto-oki M6 earthquakes. The geochemical sampling coils yielded in situ pore fluids from the splay fault zone, and microorganisms were successfully cultivated from the colonization unit. The complex sensor array, in combination with the multilevel hole completion, is one of the most ambitious and sophisticated observatory installations in scientific ocean drilling (similar to that in Hole C0002G, deployed in 2010). Overall, the installation went smoothly, efficiently, and ahead of schedule. The extra time afforded by the efficient observatory deployment was used for coring in Holes C0010B–C0010E. Despite challenging hole conditions, the depth interval corresponding to the screened casing across the megasplay fault was successfully sampled in Hole C0010C, and the footwall of the megasplay was sampled in Hole C0010E, with >50% recovery for both zones. In the hanging wall of the megasplay fault (Holes C0010C and C0010D), we recovered indurated silty clay with occasional ash layers and sedimentary breccias. Mudstones show different degrees of deformation spanning from occasional fractures to intervals of densely fractured scaly claystones of up to >10 cm thickness. Sparse faulting with low displacement (usually <2 cm) is seen in core and exhibits primarily normal and, rarely, reversed sense of slip. When present, ash was entrained along fractures and faults. In Hole C0010E, the footwall to the megasplay fault was recovered. Sediments are horizontally to gently dipping and mainly comprise silt of olive-gray color. The hanging wall sediments recovered in Holes C0010C–C0010D range in age from 3.79 to 5.59 Ma and have been thrust over the younger footwall sediments in Hole C0010E, ranging in age from 1.56 to 1.67 Ma. The deposits of the underthrust sediment prism are less indurated than the hanging wall mudstones and show lamination on a centimeter scale. The material is less intensely deformed than the mudstones, and apart from occasional fracturation (some of it being drilling disturbance), evidence of structural features is absent.more » « less
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Abstract We use in situ measurements of suspended mud to assess the flocculation state of the lowermost freshwater reaches of the Mississippi River. The goal of the study was to assess the flocculation state of the mud in the absence of seawater, the spatial distribution of floc sizes within the river, and to look for seasonal differences between summer and winter. We also examine whether measured floc sizes can explain observed vertical distributions of mud concentration through a Rouse profile analysis. Data were collected at the same locations during summer and winter at similar discharges and suspended sediment concentrations. Measurements showed that the mud in both seasons was flocculated and that the floc size could reasonably be represented by a cross‐sectional averaged value as sizes varied little over the flow depth or laterally across the river at a given station. Depth‐averaged floc sizes ranged from 75 to 200 microns and increased slightly moving downriver as turbulence levels dropped. On average, flocs were 40 microns larger during summer than in winter, likely due to enhanced microbial activity associated with warmer water. Floc size appeared to explain vertical variations in mud concentration profiles when the bed was predominately composed of sand. Average mud settling velocities for these cases ranged from 0.1 to 0.5 mm/s. However, Rouse‐estimated settling velocities ranged from 1 to 3 mm/s at two stations during winter where the bed was composed of homogeneous mud. These values exceeded the size‐based estimates of settling velocity.
