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  • Molecular phylogeny of Spirotrichonymphea (Parabasalia) with emphasis on Spironympha , Spirotrichonympha , and three new genera Pseudospironympha , Nanospironympha , and Brugerollina
Title: Molecular phylogeny of Spirotrichonymphea (Parabasalia) with emphasis on Spironympha , Spirotrichonympha , and three new genera Pseudospironympha , Nanospironympha , and Brugerollina
Abstract Spirotrichonymphea, one of the six classes of phylum Parabasalia, are characterized by bearing many flagella in spiral rows, and they occur exclusively in the guts of termites. Phylogenetic relationships among the 13 described genera are not well understood due to complex morphological evolution and a paucity of molecular data. One such understudied genus isSpironympha. It has been variously considered a valid genus, a subgenus ofSpirotrichonympha, or an “immature” life cycle stage ofSpirotrichonympha. To clarify this, we sequenced the small subunit rRNA gene sequences ofSpironymphaandSpirotrichonymphacells isolated from the hindguts ofReticulitermesspecies andHodotermopsis sjostedtiand confirmed the molecular identity ofH. sjostedtisymbionts using fluorescence in situ hybridization.Spironymphaas currently circumscribed is polyphyletic, with bothH. sjostedtisymbiont species branching separately from the “true”SpironymphafromReticulitermes. Similarly, theSpirotrichonymphasymbiont ofH. sjostedtibranches separately from the “true”Spirotrichonymphafound inReticulitermes. Our data supportSpironymphafromReticulitermesas a valid genus most closely related toSpirotrichonympha, though its monophyly and interspecific relationships are not resolved in our molecular phylogenetic analysis. We propose three new genera to accommodate theH. sjostedtisymbionts and two new species ofSpirotrichonymphafromReticulitermes.  more » « less
Award ID(s):
2045329
PAR ID:
10466907
Author(s) / Creator(s):
; ; ; ; ; ; ; ;
Publisher / Repository:
Wiley
Date Published:
Journal Name:
Journal of Eukaryotic Microbiology
Volume:
70
Issue:
3
ISSN:
1066-5234
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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