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Abstract PremiseA family‐specific probe set for sunflowers, Compositae‐1061, enables family‐wide phylogenomic studies and investigations at lower taxonomic levels, but may lack resolution at genus to species levels, especially in groups complicated by polyploidy and hybridization. MethodsWe developed a Hyb‐Seq probe set, Compositae‐ParaLoss‐1272, that targets orthologous loci in Asteraceae. We tested its efficiency across the family by simulating target enrichment sequencing in silico. Additionally, we tested its effectiveness at lower taxonomic levels in the historically complex genusPackera. We performed Hyb‐Seq with Compositae‐ParaLoss‐1272 for 19Packerataxa that were previously studied using Compositae‐1061. The resulting sequences from each probe set, plus a combination of both, were used to generate phylogenies, compare topologies, and assess node support. ResultsWe report that Compositae‐ParaLoss‐1272 captured loci across all tested Asteraceae members, had less gene tree discordance, and retained longer loci than Compositae‐1061. Most notably, Compositae‐ParaLoss‐1272 recovered substantially fewer paralogous sequences than Compositae‐1061, with only ~5% of the recovered loci reporting as paralogous, compared to ~59% with Compositae‐1061. DiscussionGiven the complexity of plant evolutionary histories, assigning orthology for phylogenomic analyses will continue to be challenging. However, we anticipate Compositae‐ParaLoss‐1272 will provide improved resolution and utility for studies of complex groups and lower taxonomic levels in the sunflower family.
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Compositae in a Crate: an outreach initiative to promote plant awareness
Studies on the perception of animals and plants often report that students prefer to learn more about animals than plants and usually have more difficultly noticing plants in the environment. This could impact conservation programs and initiatives, as animals are often considered more important and may be deemed worthier of conservation efforts. Providing students an opportunity to connect to plants and understand how they affect their lives is one step toward raising awareness around this issue. Compositae, also known as Asteraceae or the sunflower family, is the largest family of flowering plants, accounting for ~10% of flowering plant diversity in the world. They are present in virtually all biomes and environments and fulfill multiple ecological niches. Some members of the family are widely cultivated as crops, such as sunflower, lettuce, artichoke, and chicory; several species are grown as ornamental plants; and others are used in the pharmaceutical and chemical industry. Compositae’s presence in so many contexts make the family a good candidate for activities aiming to increase plant awareness, as several Compositae species are already present in our everyday lives. Here we present Compositae in a Crate, a teacher-guided outreach activity aimed at the 4th and 5th school grades. This crate of activities contains four different modules focusing on different aspects of the sunflower family: biodiversity, morphology, society, and genomics. Each module has different learning objectives and can be used independently from each other. A guide and different activities, such as flash cards, puzzles, and 3D models, are provided in each module. The activities will be prepared to meet the national and state of Tennessee education standards for the 4th and 5th grades. The produced crates will be available at different institutions and all materials will be made available online so other interested parties can produce their own crates.
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- Award ID(s):
- 2214472
- PAR ID:
- 10470637
- Publisher / Repository:
- Association of Southeastern Biologists, Inc.
- Date Published:
- Volume:
- 70
- Issue:
- 1
- ISSN:
- 1533-8436
- Page Range / eLocation ID:
- 133
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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