Study of nucleation and growth dynamic events of cubic-phase ice crystals at TiO2–water nanointerface.
more » « less Accumulation of inorganic phosphate (P We showed that P Pre‐treatment of permeabilized myofibres with 2 m The biphasic dependence of Ca
Fatiguing exercise causes hydrolysis of phosphocreatine, increasing the intracellular concentration of inorganic phosphate (P
Association of plasma membrane BKCachannels with BK‐β subunits shapes their biophysical properties and physiological roles; however, functional modulation of the mitochondrial BKCachannel (mitoBKCa) by BK‐β subunits is not established. MitoBKCa‐α and the regulatory BK‐β1 subunit associate in mouse cardiac mitochondria. A large fraction of mitoBKCadisplay properties similar to that of plasma membrane BKCawhen associated with BK‐β1 (left‐shifted voltage dependence of activation, In BK‐β1 knockout mice, cardiac mitoBKCadisplayed a low Co‐expression of BKCawith the BK‐β1 subunit in HeLa cells doubled the density of BKCain mitochondria. The present study supports the view that the cardiac mitoBKCachannel is functionally modulated by the BK‐β1 subunit; proper targeting and activation of mitoBKCashapes mitochondrial Ca2+handling.
Association of the plasma membrane BKCachannel with auxiliary BK‐β1–4 subunits profoundly affects the regulatory mechanisms and physiological processes in which this channel participates. However, functional association of mitochondrial BK (mitoBKCa) with regulatory subunits is unknown. We report that mitoBKCafunctionally associates with its regulatory subunit BK‐β1 in adult rodent cardiomyocytes. Cardiac mitoBKCais a calcium‐ and voltage‐activated channel that is sensitive to paxilline with a large conductance for K+of 300 pS. Additionally, mitoBKCadisplays a high open probability (
There is a strong impetus to establish a circular phosphorus economy by securing internally renewable phosphate (Pi) resources for use as agricultural fertilizers. Reversible Piadsorption technologies such as ion exchange can remove and recover Pifrom water/wastewater for reuse. However, existing reversible adsorbents cannot effectively discriminate against arsenate (As(V)) due to the similarity between As(V) and Pichemical structure. If As(V) is co‐recovered with Pi, the value of the recovered products for agricultural reuse is low. The objective of this study was to construct an immobilized phosphate‐binding protein (PBP)‐based Piremoval and recovery system and analyze its selectivity for Piadsorption in the presence of As(V). A range of conditions was tested, including independent, sequential, and simultaneous exposure of the two oxyanions to immobilized PBP (PBP resin). The purity of the recovered Piproduct was assessed after inducing controlled desorption of the adsorbed oxyanions at high pH (pH 12.5). Piconstituted more than 97% of the adsorbed oxyanions in the recovered product, even when As(V) was initially present at twofold higher concentrations than Pi. Therefore, PBP resin has potential to selectively remove Pi, as well as release high‐purity Pifree of As(V) contamination suitable for subsequent agricultural reuse.
Existing reversible phosphate (Pi) adsorbents cannot effectively discriminate against arsenate (As(V)) due to the similarity in their chemical structure. Co‐recovery of As(V) with Pican reduce the recovered product's reuse as a fertilizer. An immobilized phosphate‐binding protein (PBP)‐based system can be highly selective for Pieven in the presence of As(V). Piconstituted more than 97% of the recovered product, even when As(V) was present at 2‐fold higher concentrations than Pi. Immobilized PBP offers advantages over existing Piadsorbents by providing high‐purity Piproducts free of As(V) contamination for reuse.
Cardiac MR fingerprinting (cMRF) is a novel technique for simultaneous T1and T2mapping.
To compare T1/T2measurements, repeatability, and map quality between cMRF and standard mapping techniques in healthy subjects.
Prospective.
In all, 58 subjects (ages 18–60).
cMRF, modified Look–Locker inversion recovery (MOLLI), and T2‐prepared balanced steady‐state free precession (bSSFP) at 1.5T.
T1/T2values were measured in 16 myocardial segments at apical, medial, and basal slice positions. Test–retest and intrareader repeatability were assessed for the medial slice. cMRF and conventional mapping sequences were compared using ordinal and two alternative forced choice (2AFC) ratings.
Paired
Average T1measurements were: basal 1007.4
This work reports on myocardial T1/T2measurements in healthy subjects using cMRF and standard mapping sequences. cMRF had slightly lower precision, similar test–retest and intrareader repeatability, and higher scores for map quality.
2
Stage 1 J. Magn. Reson. Imaging 2020;52:1044–1052.
Self‐sustaining photocatalytic NO3−reduction systems could become ideal NO3−removal methods. Developing an efficient, highly active photocatalyst is the key to the photocatalytic reduction of NO3−. In this work, we present the synthesis of Ni2P‐modified Ta3N5(Ni2P/Ta3N5), TaON (Ni2P/TaON), and TiO2(Ni2P/TiO2). Starting with a 2 mM (28 g/mL NO3−−N) aqueous solution of NO3−, as made Ni2P/Ta3N5and Ni2P/TaON display as high as 79% and 61% NO3−conversion under 419 nm light within 12 h, which correspond to reaction rates per gram of 196 μmol g−1 h−1and 153 μmol g−1 h−1, respectively, and apparent quantum yields of 3–4%. Compared to 24% NO3−conversion in Ni2P/TiO2, Ni2P/Ta3N5and Ni2P/TaON exhibit higher activities due to the visible light active semiconductor (SC) substrates Ta3N5and TaON. We also discuss two possible electron migration pathways in Ni2P/semiconductor heterostructures. Our experimental results suggest one dominant electron migration pathway in these materials, namely: Photo‐generated electrons migrate from the semiconductor to co‐catalyst Ni2P, and upshift its Fermi level. The higher Fermi level provides greater driving force and allows NO3−reduction to occur on the Ni2P surface.
