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1. CRISPR‐Cas9 Genome Editing in the Moss Physcomitrium (Formerly Physcomitrella ) patens

   https://doi.org/10.1002/cpz1.725  

   Wu, Shu‐Zon ; Ryken, Samantha E. ; Bezanilla, Magdalena ( April 2023 , Current Protocols)

   Until recently, precise genome editing has been limited to a few organisms. The ability of Cas9 to generate double stranded DNA breaks at specific genomic sites has greatly expanded molecular toolkits in many organisms and cell types. Before CRISPR‐Cas9 mediated genome editing,P. patenswas unique among plants in its ability to integrate DNA via homologous recombination. However, selection for homologous recombination events was required to obtain edited plants, limiting the types of editing that were possible. Now with CRISPR‐Cas9, molecular manipulations inP. patenshave greatly expanded. This protocol describes a method to generate a variety of different genome edits. The protocol describes a streamlined method to generate the Cas9/sgRNA expression constructs, design homology templates, transform, and quickly genotype plants. © 2023 Wiley Periodicals LLC.

   Basic Protocol 1: Constructing the Cas9/sgRNA transient expression vector

   Alternate Protocol 1: Shortcut to generating single and pooled Cas9/sgRNA expression vectors

   Basic Protocol 2: Designing the oligonucleotide‐based homology‐directed repair (HDR) template

   Alternate Protocol 2: Designing the plasmid‐based HDR template

   Basic Protocol 3: Inducing genome editing by transforming CRISPR vector intoP. patensprotoplasts

   Basic Protocol 4: Identifying edited plants.

    

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2. Extended Ladder‐Type Benzo[ k ]tetraphene‐Derived Oligomers

   https://doi.org/10.1002/ange.201707595  

   Lee, Jongbok ; Li, Huanbin ; Kalin, Alexander J. ; Yuan, Tianyu ; Wang, Chenxu ; Olson, Troy ; Li, Hanying ; Fang, Lei ( October 2017 , Angewandte Chemie)

   Well‐defined, fused‐ring aromatic oligomers represent promising candidates for the fundamental understanding and application of advanced carbon‐rich materials, though bottom‐up synthesis and structure–property correlation of these compounds remain challenging. In this work, an efficient synthetic route was employed to construct extended benzo[k]tetraphene‐derived oligomers with up to 13 fused rings. The molecular and electronic structures of these compounds were clearly elucidated. Precise correlation of molecular sizes and crystallization dynamics was established, thus demonstrating the pivotal balance between intermolecular interaction and molecular mobility for optimized processing of highly ordered solids of these extended conjugated molecules.

    

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3. Extended Ladder‐Type Benzo[ k ]tetraphene‐Derived Oligomers

   https://doi.org/10.1002/anie.201707595  

   Lee, Jongbok ; Li, Huanbin ; Kalin, Alexander J. ; Yuan, Tianyu ; Wang, Chenxu ; Olson, Troy ; Li, Hanying ; Fang, Lei ( October 2017 , Angewandte Chemie International Edition)

   Well‐defined, fused‐ring aromatic oligomers represent promising candidates for the fundamental understanding and application of advanced carbon‐rich materials, though bottom‐up synthesis and structure–property correlation of these compounds remain challenging. In this work, an efficient synthetic route was employed to construct extended benzo[k]tetraphene‐derived oligomers with up to 13 fused rings. The molecular and electronic structures of these compounds were clearly elucidated. Precise correlation of molecular sizes and crystallization dynamics was established, thus demonstrating the pivotal balance between intermolecular interaction and molecular mobility for optimized processing of highly ordered solids of these extended conjugated molecules.

    

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4. Deciphering I–V characteristics in molecular electronics with the benefit of an analytical model

   https://doi.org/10.1039/d3cp03877g  

   Taherinia, Davood ; Frisbie, C Daniel ( December 2023 , Physical Chemistry Chemical Physics)

   The current–voltage behavior of molecular tunnel junctions can be analyzed with a simple model to reveal key electronic structure characteristics.

    

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5. Molecular phylogeny of Spirotrichonymphea (Parabasalia) with emphasis on Spironympha , Spirotrichonympha , and three new genera Pseudospironympha , Nanospironympha , and Brugerollina

   https://doi.org/10.1111/jeu.12967  

   Noda, Satoko ; Kitade, Osamu ; Jasso‐Selles, Daniel E. ; Taerum, Stephen J. ; Takayanagi, Miki ; Radek, Renate ; Lo, Nathan ; Ohkuma, Moriya ; Gile, Gillian H. ( February 2023 , Journal of Eukaryotic Microbiology)

   Spirotrichonymphea, one of the six classes of phylum Parabasalia, are characterized by bearing many flagella in spiral rows, and they occur exclusively in the guts of termites. Phylogenetic relationships among the 13 described genera are not well understood due to complex morphological evolution and a paucity of molecular data. One such understudied genus isSpironympha. It has been variously considered a valid genus, a subgenus ofSpirotrichonympha, or an “immature” life cycle stage ofSpirotrichonympha. To clarify this, we sequenced the small subunit rRNA gene sequences ofSpironymphaandSpirotrichonymphacells isolated from the hindguts ofReticulitermesspecies andHodotermopsis sjostedtiand confirmed the molecular identity ofH. sjostedtisymbionts using fluorescence in situ hybridization.Spironymphaas currently circumscribed is polyphyletic, with bothH. sjostedtisymbiont species branching separately from the “true”SpironymphafromReticulitermes. Similarly, theSpirotrichonymphasymbiont ofH. sjostedtibranches separately from the “true”Spirotrichonymphafound inReticulitermes. Our data supportSpironymphafromReticulitermesas a valid genus most closely related toSpirotrichonympha, though its monophyly and interspecific relationships are not resolved in our molecular phylogenetic analysis. We propose three new genera to accommodate theH. sjostedtisymbionts and two new species ofSpirotrichonymphafromReticulitermes.

    

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