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1. CRISPR‐Cas9 Genome Editing in the Moss Physcomitrium (Formerly Physcomitrella ) patens

   https://doi.org/10.1002/cpz1.725  

   Wu, Shu‐Zon; Ryken, Samantha E.; Bezanilla, Magdalena (April 2023, Current Protocols)

   Abstract Until recently, precise genome editing has been limited to a few organisms. The ability of Cas9 to generate double stranded DNA breaks at specific genomic sites has greatly expanded molecular toolkits in many organisms and cell types. Before CRISPR‐Cas9 mediated genome editing,P. patenswas unique among plants in its ability to integrate DNA via homologous recombination. However, selection for homologous recombination events was required to obtain edited plants, limiting the types of editing that were possible. Now with CRISPR‐Cas9, molecular manipulations inP. patenshave greatly expanded. This protocol describes a method to generate a variety of different genome edits. The protocol describes a streamlined method to generate the Cas9/sgRNA expression constructs, design homology templates, transform, and quickly genotype plants. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Constructing the Cas9/sgRNA transient expression vector Alternate Protocol 1: Shortcut to generating single and pooled Cas9/sgRNA expression vectors Basic Protocol 2: Designing the oligonucleotide‐based homology‐directed repair (HDR) template Alternate Protocol 2: Designing the plasmid‐based HDR template Basic Protocol 3: Inducing genome editing by transforming CRISPR vector intoP. patensprotoplasts Basic Protocol 4: Identifying edited plants. 

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2. Epitaxial growth of the first two members of the Ba n +1In n O2.5 n +1 Ruddlesden–Popper homologous series

   https://doi.org/10.1116/6.0002205  

   Hensling, Felix_V_E; Smeaton, Michelle_A; Show, Veronica; Azizie, Kathy; Barone, Matthew_R; Kourkoutis, Lena_F; Schlom, Darrell_G (November 2022, Journal of Vacuum Science & Technology A)

   We demonstrate the epitaxial growth of the first two members, and the n=∞ member of the homologous Ruddlesden–Popper series of Ban+1InnO2.5n+1 of which the n=1 member was previously unknown. The films were grown by suboxide molecular-beam epitaxy where the indium is provided by a molecular beam of indium-suboxide [In2O (g)]. To facilitate ex situ characterization of the highly hygroscopic barium indate films, a capping layer of amorphous SiO2 was deposited prior to air exposure. The structural quality of the films was assessed by x-ray diffraction, reflective high-energy electron diffraction, and scanning transmission electron microscopy. 

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3. Molecular phylogeny of Spirotrichonymphea (Parabasalia) with emphasis on Spironympha , Spirotrichonympha , and three new genera Pseudospironympha , Nanospironympha , and Brugerollina

   https://doi.org/10.1111/jeu.12967  

   Noda, Satoko; Kitade, Osamu; Jasso‐Selles, Daniel E.; Taerum, Stephen J.; Takayanagi, Miki; Radek, Renate; Lo, Nathan; Ohkuma, Moriya; Gile, Gillian H. (February 2023, Journal of Eukaryotic Microbiology)

   Abstract Spirotrichonymphea, one of the six classes of phylum Parabasalia, are characterized by bearing many flagella in spiral rows, and they occur exclusively in the guts of termites. Phylogenetic relationships among the 13 described genera are not well understood due to complex morphological evolution and a paucity of molecular data. One such understudied genus isSpironympha. It has been variously considered a valid genus, a subgenus ofSpirotrichonympha, or an “immature” life cycle stage ofSpirotrichonympha. To clarify this, we sequenced the small subunit rRNA gene sequences ofSpironymphaandSpirotrichonymphacells isolated from the hindguts ofReticulitermesspecies andHodotermopsis sjostedtiand confirmed the molecular identity ofH. sjostedtisymbionts using fluorescence in situ hybridization.Spironymphaas currently circumscribed is polyphyletic, with bothH. sjostedtisymbiont species branching separately from the “true”SpironymphafromReticulitermes. Similarly, theSpirotrichonymphasymbiont ofH. sjostedtibranches separately from the “true”Spirotrichonymphafound inReticulitermes. Our data supportSpironymphafromReticulitermesas a valid genus most closely related toSpirotrichonympha, though its monophyly and interspecific relationships are not resolved in our molecular phylogenetic analysis. We propose three new genera to accommodate theH. sjostedtisymbionts and two new species ofSpirotrichonymphafromReticulitermes. 

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4. DNA conductance modulation via aptamer binding

   https://doi.org/10.1039/d4nr05139d  

   Mohammad, Hashem; Alsaleh, Lina; Alotaibi, Abrar; Alolaiyan, Olaiyan; Takahashi, Taisei; Anantram, M P; Nishino, Tomoaki (March 2025, Nanoscale)

   Aptamer binding to DNA increases conductance over tenfold, enabling high-resistance contrast DNA strands for molecular electronics development. 

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5. Deciphering I–V characteristics in molecular electronics with the benefit of an analytical model

   https://doi.org/10.1039/d3cp03877g  

   Taherinia, Davood; Frisbie, C Daniel (December 2023, Physical Chemistry Chemical Physics)

   The current–voltage behavior of molecular tunnel junctions can be analyzed with a simple model to reveal key electronic structure characteristics. 

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