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Title: In-focus quantitative phase imaging from defocused off-axis holograms: synergistic reconstruction framework
Digital holographic microscopy (DHM) enables the three-dimensional (3D) reconstruction of quantitative phase distributions from a defocused hologram. Traditional computational algorithms follow a sequential approach in which one first reconstructs the complex amplitude distribution and later applies focusing algorithms to provide an in-focus phase map. In this work, we have developed a synergistic computational framework to compensate for the linear tilt introduced in off-axis DHM systems and autofocus the defocused holograms by minimizing a cost function, providing in-focus reconstructed phase images without phase distortions. The proposed computational tool has been validated in defocused holograms of human red blood cells and three-dimensional images of dynamic sperm cells.  more » « less
Award ID(s):
2042563
PAR ID:
10476321
Author(s) / Creator(s):
; ;
Publisher / Repository:
Optical Society of America
Date Published:
Journal Name:
Optics Letters
Volume:
48
Issue:
23
ISSN:
0146-9592; OPLEDP
Format(s):
Medium: X Size: Article No. 6244
Size(s):
Article No. 6244
Sponsoring Org:
National Science Foundation
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    Carretero, Luis (Ed.)
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  2. ; ; (, PLOS ONE)
    Grulkowski, Ireneusz (Ed.)
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  3. ; ; (, Sensors)
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