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  • A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean
Title: A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean
Abstract BackgroundSoybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time-consuming and technically challenging. An attractive alternative is to administer transient gene silencing or overexpression with a plant virus-based vector. However, existing virus-induced gene silencing (VIGS) and/or overexpression vectors suitable for soybean have various drawbacks that hinder their widespread adoption. ResultsWe describe the development of a new vector based on cowpea severe mosaic virus (CPSMV), a plus-strand RNA virus with its genome divided into two RNA segments, RNA1 and RNA2. This vector, designated FZ, incorporates a cloning site in the RNA2 cDNA, permitting insertion of nonviral sequences. When paired with an optimized RNA1 construct, FZ readily infects bothNicotiana benthamianaand soybean. As a result, FZ constructs destined for soybean can be first delivered toN. benthamianain order to propagate the modified viruses to high titers. FZ-based silencing constructs induced robust silencing of phytoene desaturase genes inN. benthamiana, multiple soybean accessions, and cowpea. Meanwhile, FZ supported systemic expression of fluorescent proteins mNeonGreen and mCherry inN. benthamianaand soybean. Finally, FZ-mediated expression of the Arabidopsis transcription factor MYB75 causedN. benthamianato bear brown leaves and purple, twisted flowers, indicating that MYB75 retained the function of activating anthocyanin synthesis pathways in a different plant. ConclusionsThe new CPSMV-derived FZ vector provides a convenient and versatile soybean functional genomics tool that is expected to accelerate the characterization of soybean genes controlling crucial productivity traits.  more » « less
Award ID(s):
1758912
PAR ID:
10479165
Author(s) / Creator(s):
; ; ; ; ;
Publisher / Repository:
BMC
Date Published:
Journal Name:
Plant Methods
Volume:
18
Issue:
1
ISSN:
1746-4811
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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