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Title: Nondestructive 3D Pathology with Light-Sheet Fluorescence Microscopy for Translational Research and Clinical Assays
In recent years, there has been a revived appreciation for the importance of spatial context and morphological phenotypes for both understanding disease progression and guiding treatment decisions. Compared with conventional 2D histopathology, which is the current gold standard of medical diagnostics, nondestructive 3D pathology offers researchers and clinicians the ability to visualize orders of magnitude more tissue within their natural volumetric context. This has been enabled by rapid advances in tissue-preparation methods, high-throughput 3D microscopy instrumentation, and computational tools for processing these massive feature-rich data sets. Here, we provide a brief overview of many of these technical advances along with remaining challenges to be overcome. We also speculate on the future of 3D pathology as applied in translational investigations, preclinical drug development, and clinical decision-support assays.  more » « less
Award ID(s):
1934292
PAR ID:
10482702
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
PubMed
Date Published:
Journal Name:
Annual Review of Analytical Chemistry
Volume:
16
Issue:
1
ISSN:
1936-1327
Page Range / eLocation ID:
231 to 252
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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