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1. Fossil fern rhizomes as a model system for exploring epiphyte community structure across geologic time: evidence from Patagonia

   https://doi.org/10.7717/peerj.8244  

   Bippus, Alexander C. ; Escapa, Ignacio H. ; Wilf, Peter ; Tomescu, Alexandru M. F. ( December 2019 , PeerJ)

   In extant ecosystems, complex networks of ecological interactions between organisms can be readily studied. In contrast, understanding of such interactions in ecosystems of the geologic past is incomplete. Specifically, in past terrestrial ecosystems we know comparatively little about plant biotic interactions besides saprotrophy, herbivory, mycorrhizal associations, and oviposition. Due to taphonomic biases, epiphyte communities are particularly rare in the plant-fossil record, despite their prominence in modern ecosystems. Accordingly, little is known about how terrestrial epiphyte communities have changed across geologic time. Here, we describe a tinyin situfossil epiphyte community that sheds light on plant-animal and plant-plant interactions more than 50 million years ago.

   A single silicifiedTodea(Osmundaceae) rhizome from a new locality of the early Eocene (ca. 52 Ma) Tufolitas Laguna del Hunco (Patagonia, Argentina) was studied in serial thin sections using light microscopy. The community of organisms colonizing the tissues of the rhizome was characterized by identifying the organisms and mapping and quantifying their distribution. A 200 × 200 µm grid was superimposed onto the rhizome cross section, and the colonizers present at each node of the grid were tallied.

   Preservedin situ, this community offers a rare window onto aspects of ancient ecosystems usually lost to time and taphonomic processes. The community is surprisingly diverse and includes the first fossilized leafy liverworts in South America, also marking the only fossil record of leafy bryophyte epiphytes outside of amber deposits; as well as several types of fungal hyphae and spores; microsclerotia with possible affinities in several ascomycete families; and evidence for oribatid mites.

   The community associated with the Patagonian rhizome enriches our understanding of terrestrial epiphyte communities in the distant past and adds to a growing body of literature on osmundaceous rhizomes as important hosts for component communities in ancient ecosystems, just as they are today. Because osmundaceous rhizomes represent an ecological niche that has remained virtually unchanged over time and space and are abundant in the fossil record, they provide a paleoecological model system that could be used to explore epiphyte community structure through time.

    

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2. Maximizing Molecular Data From Low-Quality Fluid-Preserved Specimens in Natural History Collections

   https://doi.org/10.3389/fevo.2022.893088  

   Bernstein, Justin M. ; Ruane, Sara ( June 2022 , Frontiers in Ecology and Evolution)

   Over the past decade, museum genomics studies have focused on obtaining DNA of sufficient quality and quantity for sequencing from fluid-preserved natural history specimens, primarily to be used in systematic studies. While these studies have opened windows to evolutionary and biodiversity knowledge of many species worldwide, published works often focus on the success of these DNA sequencing efforts, which is undoubtedly less common than obtaining minimal or sometimes no DNA or unusable sequence data from specimens in natural history collections. Here, we attempt to obtain and sequence DNA extracts from 115 fresh and 41 degraded samples of homalopsid snakes, as well as from two degraded samples of a poorly known snake, Hydrablabes periops . Hydrablabes has been suggested to belong to at least two different families (Natricidae and Homalopsidae) and with no fresh tissues known to be available, intractable museum specimens currently provide the only opportunity to determine this snake’s taxonomic affinity. Although our aim was to generate a target-capture dataset for these samples, to be included in a broader phylogenetic study, results were less than ideal due to large amounts of missing data, especially using the same downstream methods as with standard, high-quality samples. However, rather than discount results entirely, we used mapping methods with references and pseudoreferences, along with phylogenetic analyses, to maximize any usable molecular data from our sequencing efforts, identify the taxonomic affinity of H. periops , and compare sequencing success between fresh and degraded tissue samples. This resulted in largely complete mitochondrial genomes for five specimens and hundreds to thousands of nuclear loci (ultra-conserved loci, anchored-hybrid enrichment loci, and a variety of loci frequently used in squamate phylogenetic studies) from fluid-preserved snakes, including a specimen of H. periops from the Field Museum of Natural History collection. We combined our H. periops data with previously published genomic and Sanger-sequenced datasets to confirm the familial designation of this taxon, reject previous taxonomic hypotheses, and make biogeographic inferences for Hydrablabes . A second H. periops specimen, despite being seemingly similar for initial raw sequencing results and after being put through the same protocols, resulted in little usable molecular data. We discuss the successes and failures of using different pipelines and methods to maximize the products from these data and provide expectations for others who are looking to use DNA sequencing efforts on specimens that likely have degraded DNA. Life Science Identifier ( Hydrablabes periops ) urn:lsid:zoobank.org :pub:F2AA44 E2-D2EF-4747-972A-652C34C2C09D. 

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3. Using Leaf δ13C to Assess Water Use Efficiency within Plant Communities Across a Successional Gradient in Temperate and Tropical Forests

   Meier, Josephine ; Lowe, Alexander ; Both, Sabine ; Oishi, A C ; Aguirre, E ; Strömberg, C A ( July 2023 , Botany)

   Understanding how plant communities of the past have responded to disturbance events can provide valuable insights when managing our natural resources and assessing human impacts on ecosystems. The geologic record has the potential to reflect these responses through the analysis of functional traits, which relate directly to plant function and ecosystem strategy. There is currently little evidence of how functional traits measurable in fossil leaves vary across succession in different forest types. Because of this, there is a limited ability to identify disturbance as the primary driver of vegetation change within the fossil record. To improve this ability, this study analyzes the carbon stable isotopic composition (δ 13C) of bulk organic matter sampled at the community-scale across successional gradients in a temperate deciduous forest (North Carolina, USA) and compares them against values from a previous study across succession in a tropical evergreen forest (Malaysian Borneo). Leaf δ13C is representative of a plant's water use efficiency (WUE), an important axis of ecological strategy representing the carbon assimilated per water lost in a plant during photosynthesis. Leaf δ13C as a functional trait has the advantage that it is often preserved during leaf fossilization and, integrated across a plant community, can be informative about prevalent ecological strategies, functional diversity , and community assembly dynamics. In Borneo, the community-weighted mean of leaf δ13C to be highest in early-succession plots, indicative of a higher WUE in plant communities closely following a disturbance event. Old growth plots were found to have a lower δ13C, and thus a more conservative WUE. This study will further investigate if this trend is followed within temperate forests, which is important as many mid-late Cenozoic plant assemblages come from what would have been temperate regions. Developing a method of identifying disturbances within the geologic record, will improve the ability to discern drivers of plant community change in the past. This improved knowledge will help guide management decisions across a range of ecosystems. 

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4. Distinctive microfossil supports early Paleoproterozoic rise in complex cellular organisation

   https://doi.org/10.1111/gbi.12576  

   Barlow, Erica V. ; House, Christopher H. ; Liu, Ming‐Chang ; Wetherington, Maxwell T. ; Van Kranendonk, Martin J. ( October 2023 , Geobiology)

   The great oxidation event (GOE), ~2.4 billion years ago, caused fundamental changes to the chemistry of Earth's surface environments. However, the effect of these changes on the biosphere is unknown, due to a worldwide lack of well‐preserved fossils from this time. Here, we investigate exceptionally preserved, large spherical aggregate (SA) microfossils permineralised in chert from the c. 2.4 Ga Turee Creek Group in Western Australia. Field and petrographic observations, Raman spectroscopic mapping, and in situ carbon isotopic analyses uncover insights into the morphology, habitat, reproduction and metabolism of this unusual form, whose distinctive, SA morphology has no known counterpart in the fossil record. Comparative analysis with microfossils from before the GOE reveals the large SA microfossils represent a step‐up in cellular organisation. Morphological comparison to extant micro‐organisms indicates the SAs have more in common with coenobial algae than coccoidal bacteria, emphasising the complexity of this microfossil form. The remarkable preservation here provides a unique window into the biosphere, revealing an increase in the complexity of life coinciding with the GOE.

    

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5. Rhabdopleurid epibionts from the Ordovician Fezouata Shale biota and the longevity of cross-phylum interactions

   https://doi.org/10.1038/s42003-023-05377-x  

   Nanglu, Karma ; Waskom, Madeleine E. ; Richards, Jared C. ; Ortega-Hernández, Javier ( October 2023 , Communications Biology)

   Evidence of interspecific interactions in the fossil record is rare but offers valuable insights into ancient ecologies. Exceptional fossiliferous sites can preserve complex ecological interactions involving non-biomineralized organisms, but most of these examples are restricted to Cambrian Lagerstätten. Here we report an exceptionally preserved cross-phylum interspecific interaction from the Tremadocian-aged Lower Fezouata Shale Formation of Morocco, which consists of the phragmocone of an orthocone cephalopod that has been extensively populated post-mortem by tubicolous epibionts. Well-preserved transverse bands in a zig-zag pattern and crenulations along the margin of the unbranched tubes indicate that they correspond to pterobranch hemichordates, with a close morphological similarity to rhabdopleurids based on the bush-like growth of the dense tubarium. The discovery of rhabdopleurid epibionts in the Fezouata Shale highlights the paucity of benthic graptolites, which also includes the rooted dendroidsDidymograptusandDictyonema, relative to the substantially more diverse and abundant planktic forms known from this biota. We propose that the rarity of Paleozoic rhabdopleurid epibionts is likely a consequence of their ecological requirement for hard substrates for initial settlement and growth. The Fezouata rhabdopleurid also reveals a 480-million-year-old association of pterobranchs as epibionts of molluscs that persist to the present day.

    

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