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  • Fluid Flow Analysis for Suitable 3D Bio-Printed Scaffold Architectures to Incubate in a Perfusion Bioreactor: A Simulation Approach
Title: Fluid Flow Analysis for Suitable 3D Bio-Printed Scaffold Architectures to Incubate in a Perfusion Bioreactor: A Simulation Approach
Abstract

Due to the three-dimensional nature of the 3D bio-printed scaffolds, typical stagnant cell culturing methods don’t ensure entering medium inside areas or passing through the scaffolds. The bioreactor has frequently provided the required growth medium to encapsulated- and seeded-cells in 3D bio-printed scaffolds. To address this issue, we developed a customized perfusion bioreactor to supply the growth medium dynamically to the cells encapsulated or seeded in the scaffolds. The dynamic supply of fresh growth medium may help improve cell viability and proliferation. Because of its uniform nutrition distribution and flow-induced shear stress within the tissue-engineering scaffold, perfusion bioreactors have been used in a variety of tissue engineering applications. Including a modified setup of our designed bioreactor may improve the in vivo stimuli and conditions, eventually enhancing the overall performance of tissue regeneration. In this paper, we explored the response of fluid flow to certain types of scaffold pore geometries and porosities. We used a simulation technique to determine fluid flow turbulence through various pore geometries such as uniform triangular, square, diamond, circular, and honeycomb. We used variable pore sizes of the scaffold maintaining constant porosity to analyze the fluid flow. Based on the results, optimum designs for scaffolds were determined.

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Award ID(s):
1757371
NSF-PAR ID:
10486635
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
American Society of Mechanical Engineers
Date Published:
Journal Name:
Proceedings of the ASME 2023 18th International Manufacturing Science and Engineering Conference (MSEC2023)
ISBN:
978-0-7918-8723-3
Format(s):
Medium: X
Location:
New Brunswick, New Jersey, USA
Sponsoring Org:
National Science Foundation
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