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This content will become publicly available on September 20, 2024

Title: High‐throughput fabrication, structural characterization, and cellular interaction of compositionally diverse fish gelatin/polycaprolactone ( PCL ) nanofibrous materials
Abstract

Nanofibers made by blending natural and synthetic biopolymers have shown promise for better mechanical stability, ECM morphology mimicry, and cellular interaction of such materials. With the evolution of production methods of nanofibers, alternating field electrospinning (a.k.a. alternating current (AC) electrospinning) demonstrates a strong potential for scalable and sustainable fabrication of nanofibrous materials. This study focuses on AC‐electrospinning of poorly miscible blends of gelatin from cold water fish skin (FGEL) and polycaprolactone (PCL) in a range of FGEL/PCL mass ratios from 0.9:0.1 to 0.4:0.6 in acetic acid single‐solvent system. The nanofiber productivity rates of 7.8–19.0 g/h were obtained using a single 25 mm diameter dish‐like spinneret, depending on the precursor composition. The resulting nanofibrous meshes had 94%–96% porosity and revealed the nanofibers with 200–750 nm diameters and smooth surface morphology. The results of FTIR, XRD, and water contact angle analyses have shown the effect of FGEL/PCL mass ratio on the changes in the material wettability, PCL crystallinity and orientation of PCL crystalline regions, and secondary structure of FGEL in as‐spun and thermally crosslinked materials. Preliminary in vitro tests with 3 T3 mouse fibroblasts confirmed favorable and tunable cell attachment, proliferation, and spreading on all tested FGEL/PCL nanofibrous meshes.

 
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Award ID(s):
1852207
NSF-PAR ID:
10489734
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
Wiley
Date Published:
Journal Name:
Journal of Applied Polymer Science
Volume:
140
Issue:
36
ISSN:
0021-8995
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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